Predict the phenotype of a lac Y mutant, which is a mutation in the gene for...
Predict the phenotype of a lac Y mutant, which is a mutation in the gene for lactose permease.
a. The lac genes would be expressed efficiently only in the absence of lactose.
b. The lac genes would be expressed efficiently until the lactose supply already in the cell is exhausted.
c. The lac genes would be expressed continuously.
d. Expression of the lac genes would cease immediately.
Homework Answers
The correct answer is:
b. The lac genes would be expressed efficiently until the lactose supply already in the cell is exhausted.
Lactose cannot enter cell if there is no lactose permease as because the lactose permease is involved with the transport of lactose into the cell through the cell membrane
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Predict the phenotype of a lac Y mutant, which is a mutation in
the gene for...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense mutation in the DNA binding domain of the protein. IN can not bind to the operator. Predict the outcome of the following lac operon genotype for the expression of functional lac enzymes in the different conditions indicated. (Note: + = wildtype, - = no functional protein made.) IN O+P+ Z-Y+ - lactose + lactose b-gal (Z) Permease (Y) b-gal (Z) Permease (Y) + +...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon of E. coli leads to an inability to ferment lactose, and the expression of the operon is always off. Introduction into the mutant of an F' factor containing the wild type lac operon does NOT restore the ability to ferment lactose, i.e., there is no expression of the operon from the plasmid either. a. What is the probable nature of Mutation A? Is the...
Please solve all of them Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP...
Please solve all of them
Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP acolacZacY Carbon source in theLac operon p-galactosidaseLactose permease binding site me levels in levels in the cell polycistronic mRNA enzy levels in the cell Undetectable Low but detectable Low but deteteable Low but detectable medium the cell Undetectable membrane Glucose only Glucose and lactose Lactose on Another carbon source Undetectable Undetectable Undetectable Undetectable The table shows the results of experiments measuring expression of the...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site in DNA, thus allowing for expression of the genes coded by the Lac Operon. However, lactose cannot cross the bacterial cell membrane without the help of the Permease protein channel, which is coded by the lacY gene in the Lac Operon. So, it appears that we have a case of "what's first, the chicken or the egg?" - how can lactose enter the cell...
A lac operon mutant is grown in the presence and absence of lactose and tested for...
A lac operon mutant is grown in the presence and absence of lactose and tested for activity of the biosynthesis genes ‘Z’, ‘Y’, and ‘A’. Given the genotype below, which biosynthetic genes would be expressed in the presence of lactose? I+ P+ O+ Z+ Y- A+/ F’ I- P+ O+ Z+ Y- A+ Select one: a. none b. Z, Y, and A c. P and O d. Z and A e. I, P, O, Z, Y, and A
A constitutive mutant is a strain that had a mutation in an operon leading to that...
A constitutive mutant is a strain that had a mutation in an operon leading to that strain continuously making protein (or continuous gene expression). This operon is inducible in the wild type strain (no mutation) and therefore regulated. What would be two possible explanations on how an inducible operon would become constitutive? (hints: think about the different mechanisms of induction and then consider what would happen is these components had mutations) With regard to the Lac operon, if a bacterium goes from a...
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following...
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following statements is consistent with the expression phenotype? genome: I+P+ O- Z+ Y- plasmid: I+ P- O+ Z- Y+ constitutive expression of β-gal and permease induced in the presence of lactose constitutive expression of β-gal only constitutive expression of β-gal and permease β-gal and permease induced in the presence of lactose constitutive expression of permease and β-gal induced in the presence of lactose
Imagine you are carrying out research on the lac operon. You isolate six mutations in the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for wild type; P– for mutant (RNA polymerase does not bind)) that serves as the RNA polymerase binding site. The lac operon also contains a DNA sequence known as the Lac operator (O or O+ for wild type; O– or Oc for mutant (lac repressor cannot bind)) which is the binding site for lac repressor. The lac repressor, a protein, is encoded by the lac...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts the function of each of the following elements: a. the promoter for LacI (P(I)) b. the LacI gene c. CRP binding site d. the promoter for the lac operon (P(lac)) e. the operator sequence f. a mutation in lacZ that disrupts the coding region but does not disrupt transcription g. a mutation in lacZ that blocks transcription For each of the above mutations, what...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense mutation in the DNA binding domain of the protein. IN can not bind to the operator. Predict the outcome of the following lac operon genotype for the expression of functional lac enzymes in the different conditions indicated. (Note: + = wildtype, - = no functional protein made.) IN O+P+ Z-Y+ - lactose + lactose b-gal (Z) Permease (Y) b-gal (Z) Permease (Y) + +...
Please solve all of them
Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP acolacZacY Carbon source in theLac operon p-galactosidaseLactose permease binding site me levels in levels in the cell polycistronic mRNA enzy levels in the cell Undetectable Low but detectable Low but deteteable Low but detectable medium the cell Undetectable membrane Glucose only Glucose and lactose Lactose on Another carbon source Undetectable Undetectable Undetectable Undetectable The table shows the results of experiments measuring expression of the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
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