Homework Answers
Answer:
d. Taq polymerase
Explanation:
The primers represent the starting point for the next step, called the extension step. During this step, Taq polymerase binds to each PCR primer and begins adding nucleotides.
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Which of the following is involved in the extension step of PCR? a. DNA gyrase b....
Which of the following enzymes is involved in extending the ends of linear chromosomal DNA? a....
Which of the following enzymes is involved in extending the ends of linear chromosomal DNA? a. telomerase b. ligase c. primase d. RNA polymerase e. DNA polymerase a
Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA...
Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA ligase used is from a thermophilic bacteria so that it can resist denaturation at high temperatures. b Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. c The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis. d DNA amplified by PCR can be cloned.
49. Which one of these enzymes is involved in the process of transcription? helicase primase gyrase...
49. Which one of these enzymes is involved in the process of transcription? helicase primase gyrase DNA polymerase RNA polymerase nearumning genes have at their terminals
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in...
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in the replication of E. coli DNA. What features of the E. coli replisome and of DNA polymerase III’s structure are associated with replication processivity? How do mammalian cells prime discontinuous strand replication and how do they remove RNA primers?
Which of the following is needed for the PCR technique to work? (multiple answers) a. A...
Which of the following is needed for the PCR technique to work? (multiple answers) a. A probe that can anneal to the center of the target DNA sequence. b. A thermostable RNA polymerase c. Large amounts of pure DNA d. A thermostable DNA polymerase e. Two DNA primers that can anneal to the target DNA on either side of the DNA to be amplified. f. A thermostable DNA ligase
Which of the following components is NOT used in the technique of PCR to amplify a...
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
Which items below are involved in primer removal and replacement? Choose all that apply from the...
Which items below are involved in primer removal and replacement? Choose all that apply from the list below. Include both prokaryotes and eukaryotes. O DNA polymerase | O DNA Polymerase III O DNA polymerase delta O DNA polymerase alpha DNA polymerase epsilon Replication protein A Primase Topoisomerase/gyrase Single Stranded binding proteins DNA ligase Initiator proteins dNTPs (deoxyribonucleotides) NTPs (ribonucleotids) ORI o da O O Telomerase
In PCR, how is the segment of DNA that is to be amplified determined? A. By...
In PCR, how is the segment of DNA that is to be amplified determined? A. By the activity of the polymerase B. By the extension of the annealed primers C. By using primers that flank the target sequence D. By denaturation of the entire genome
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR...
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR deletes DNA b. Polymerase Copying Repeats; PCR amplifies DNA c. Polymerase Copying Releats; PCR deletes DNA d. Polymerase Chain Reaction; PCR amplifies DNA 2) During gel electrophoresis, the DNA fragments are separated by ____ a. charge b. DNA fragments cannot be separated c. color d. size 3) Primers are a. double stranded DNA oligonucleotide (fragment) b. double stranded RNA oligonucleotide (fragment) c. single stranded...
1. Answer the following quick Cell Biology questions. (a.)DNA replication is ___________________. a. conservative. b. dispersive...
1. Answer the following quick Cell Biology questions. (a.)DNA replication is ___________________. a. conservative. b. dispersive c. semiconservative. d. irregular. e. none of the above (b.) Which of the following proteins is observed exclusively in association with eukaryotic DNA replication? a. DNA polymerase I b. DNA gyrase c. single-strand binding proteins d. DNA ligase e. telomerase (c.) Which of the following events is characteristic of DNA replication in prokaryotes but not in eukaryotes (nuclear DNA)? a. DNA synthesis 5'...
49. Which one of these enzymes is involved in the process of transcription? helicase primase gyrase DNA polymerase RNA polymerase nearumning genes have at their terminals
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
Which items below are involved in primer removal and replacement? Choose all that apply from the list below. Include both prokaryotes and eukaryotes. O DNA polymerase | O DNA Polymerase III O DNA polymerase delta O DNA polymerase alpha DNA polymerase epsilon Replication protein A Primase Topoisomerase/gyrase Single Stranded binding proteins DNA ligase Initiator proteins dNTPs (deoxyribonucleotides) NTPs (ribonucleotids) ORI o da O O Telomerase
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