1. Pribnow box, TATAAT, transcription
Transcription is the process of formation of using DNA as a template and enzyme DNA dependent RNA polymerase. It takes place in the cytoplasm of prokaryotes and the nucleus of eukaryotes. It begins at a specific region on DNA called as promoter where RNA polymerase and transcription factor binds to form transcription initiation complex.
Eukaryotes and archeabacteria have TATA box called as Hogness box. The consensus sequence is TATAAA.
Prokaryotes have TATA box called as Pribnow box. The consensus sequence is TATAAT.
It is present at -10 region.
2. Proteins and rRNA
Ribosomes are membraneless organelles which are common in both prokaryotes and eukaryotes. It is made up of proteins and ribosomal RNA. They are synthesized inside the nucleolus. They participate in the formation of proteins during the process of translation.
According to the guidelines, only first question can be answered. I have answered two for you.
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In prokaryotes the consensus sequence begins. is located about 10 bases upstream from the initiation site....
The macromolecular complex that associates with each intron and splices it is called a(n). splicer acrosome splice engine spliceosome splicing body Transcription in prokaryotes: 1. Requires consensus nucleotide sequences at position -35 and -10 in the promoter region of gene sequence. 2. Requires different sigma factors depending on the environmental stimulus. 3. Can produce a cDNA. All are correct. 3 1 Both 1 and 2 are correct. Which of the following statements about transcription factor TFIIH are correct: Two of...
A sequence of a eukaryotic gene (coding strand) is shown below, RNA polymerase recognizes the sequence ‘TATAAT’ and initiates transcription six nucleotides downstream of the sequence. The intron splice sites are CUU (5’ splice site) and AAG (3’ splice site), poly-A tails are added following the sequence AGUUGG. The poly-A tails are 20 nucleotides. b. If this is an oncogene that is elevated in cancer cells, design two siRNAs to knock down the mRNA, list the sequences of the siRNAs...
Please help with 1-16!!! (two pictures are attached) Thanks!
Transcription . Although both prokaryotes and eukaryotes put a cap and a tail on the mRNA, only eukaryotes have introns that have to be spliced out. (T/F) 2. The poly A tail on cukaryotic mRNA protects the RNA from rapid degradation in the cytoplasm. (T/F) 3. The polyA tail is added to eukaryotic mRNA immediatel after transport of the message from the nucleus. (T/F) 4. is usually a single stranded molecule....
Which of the following is NOT a function of transcription that requires the activity from subunits of the Core RNA Palymerase? a. RNA polymerase activity that base-pairs and polymerizes nucleotides to make mRNA. b. Helicase activity that unwinds the double-stranded DNA molecule for transcription c. Specific recognition of -35 box and -10 box sites in the promoter region. d. General binding that helps RNA polymerase loosely adhere to DNA, before Transcription begins. Oe. Trick Question. The Core RNA polymerase can...
Answer the questions:
Question 1 Transcription begins at the..... a. operon o b. repressor c. genome 17d. promoter Question 2 0.5 points Save RNA is synthesized on a DNA template in a process called replication, DNA polymerase translation, RNA polymerase transcription, RNA polymerise t ranscription, DNA polymerase Question 3 Which eukaryotic RNA polymerase makes tRNA's? a RNA polymerase IIIb. Any of these RNA polymerase I od RNA polymerase II A Moving to another question will save this response. Question 4...
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic RNA usually undergoes nuclear processing C. Polypeptides are synthesized by addition of amino acids to the amino terminus. D. The 3' end of mRNA corresponds to the carboxyl terminus of the protein. Grade 2. Which of the following A. It may be autocatalytic. B. Spliceosomes are present in organelles and nuclei C. It involves removal of exons is true regarding RNA processing? D. It...
.Select the probe sequence that will hybridize to the following nucleic acid sequence: C G A T A T T G T C A. T A G T A C A A G A B. C G A T A T T G T C C. G T C A A G A C C T D G C T A T A A C A G Select the strand of RNA that is complementary to this single strand of...
1. A sequence of a eukaryotic gene (coding strand) is shown below, RNA polymerase recognizes the sequence ‘TATAAT’ and initiates transcription six nucleotides downstream of the sequence. The in tron splice sites are CUU (5’ splice site) and AAG (3’ splice site), poly -A tails are added following the sequence AGUUGG. The poly- A tails are 20 nucleotides. a. Predict the sequence of mature mRNA and denote 5’ and 3’ ends. b. If this is an oncogene that is elevated...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in the top (5' to 3) strand. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG- 3' 3-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC- 5' Once Cas9 binds to a PAM sequence, it unwinds the DNA. If the guide RNA matches the DNA sequence next to the PAM, the guide RNA will bind to the complementary DNA strand. If not, the DNA will zip back together and Cas9 will keep binding to other PAM sequences until it finds the...
Below is a series of events involved in the mechanism of forming a retrotransposon. Place these steps in the correct order 1. the DNA copy is made double-stranded 2. DNA of the transposable element is transcribed 3. The DNA of the transposable element is integrated into a target DNA site 4. The RNA is reverse transcribed by reverse transcriptase, producing a complementary DNA 4,2,3,1 3,2,4,1 2,4,1,3 4,2,1,3 1,2,3,4 What is the function of the poly(A) tail on most mRNAs To...