Homework Answers
a. Fermentation = Anaerobic respiration
It produces ethanol in yeast.
C1 of glucose becomes the methyl group of ethanol.
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Answer any one and only one of the following: a. If glucose labeled with 14C in...
Answer any one and only one of the following: a. For each glucose molecule removed from...
Answer any one and only one of the following: a. For each glucose molecule removed from the nonreducing end of glycogen in mammalian skeletal muscle what is the theoretical maximum number of ATPs produced under anaerobic conditions b. If one molecule of eicosanoic acid (20:0) were metabolized in mammalian tissues under aerobic conditions what is the theoretical maximum number of ATPs produced
If pyruvate labeled with 14C in the 1 position were metabolized under aerobic conditions by mammalian...
If pyruvate labeled with 14C in the 1 position were metabolized under aerobic conditions by mammalian cells, what compound would be labeled in what position? OA. CO2 B. C-1 of ethanol CC-2 of acetate D.C-2 of citrate E. C-1 of oxaloacetate F. none of the above.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liv...
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liver homogenate under anaerobic conditions, which carbon in the lactate produced would be labeled with 1℃ (refer to the carboxyl carbon of lactate as C1, the central carbon as C2, and the methyl carbon as C3)? What if theglucose were labeled at carbon #3 instead? In both cases, explain your answer.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated...
If glucose labeled with 14C at C-2 were metabolized in the liver, the first radioactive pyruvate...
If glucose labeled with 14C at C-2 were metabolized in the liver, the first radioactive pyruvate formed would be labeled in (pick from list given below) i) all three carbons ii) its carbonyl carbon iii) its carboxyl carbon iv) its methyl carbon Ali B) ii C) iii D) iv E) A and C
Q4. Compound A is labeled at the methyl carbon with 14C (*). Under fasting conditions, the...
Q4. Compound A is labeled at the methyl carbon with 14C (*). Under fasting conditions, the 14C label ends up in compound C during steady-state metabolism in liver. COO- çoo- FO +H COO- H *CH3 a. What is the metabolic pathway involved? Write the reaction including all reactants and products, no structures required (1) b. Which carbons in compound C will become labeled, and to what percent? Briefly explain. (3) c. What is the physiological basis and the molecular mechanism...
In a "pulse chase" experiment under anaerobic conditions, 14C glucose is added to an ethanol-producing yeast...
In a "pulse chase" experiment under anaerobic conditions, 14C glucose is added to an ethanol-producing yeast cell extract just long enough that each intermediate in the ethanol fermentation is labeled with a 14C (pulse). The 14C label is then diluted so that no additional radioactivity is incorporated into the intermediates (chase). 1. What position in ethanol will be labeled with 14C if 14C-glucose labeled at position C-1 is used? 2. Where should the 14C label sit in glucose for it...
If the C-2 carbon of glucose were labeled with 14C, which if the carbons atoms in...
If the C-2 carbon of glucose were labeled with 14C, which if the carbons atoms in 3PG would be labeled during glycosis ? Give a numeric answer please .
Glucose labeled with 14C atom in position C-1 is incubated with glycolysis enzymes. what part (%) of...
Glucose labeled with 14C atom in position C-1 is incubated with glycolysis enzymes. what part (%) of pyruvate molecules will be labeled
You feed pigs pyruvate that is isotopically labeled with 14C only at its methyl group (all...
You feed pigs pyruvate that is isotopically labeled with 14C only at its methyl group (all other positions use standard natural abundance of 12C). Assuming that all of the pyruvate enters the TCA cycle, how many rounds of the TCA cycle are required before 14C is first released? Describe how you came to this conclusion.. Mammals lack the ability to biosynthesize glucose from acetyl-CoA, if a BALB/c mouse is given food containing 14C-labeled acetate, after some period of time it...
You are carrying out an experiment in the lab to study the citric acid cycle. To do this, you add 14C-labeled pyruvate at carbon 3 (this is the methyl carbon) to metabolically active mitochondria. Bas...
You are carrying out an experiment in the lab to study the citric acid cycle. To do this, you add 14C-labeled pyruvate at carbon 3 (this is the methyl carbon) to metabolically active mitochondria. Based on your knowledge of the citric acid cycle, what is the location of the 14C-label in oxaloacetate after one turn of the cycle? How many turns of the cycle are required to release all of the14C-label as CO2? Be sure to justify your answers by...
Answer any one and only one of the following: a. For each glucose molecule removed from the nonreducing end of glycogen in mammalian skeletal muscle what is the theoretical maximum number of ATPs produced under anaerobic conditions b. If one molecule of eicosanoic acid (20:0) were metabolized in mammalian tissues under aerobic conditions what is the theoretical maximum number of ATPs produced
If pyruvate labeled with 14C in the 1 position were metabolized under aerobic conditions by mammalian cells, what compound would be labeled in what position? OA. CO2 B. C-1 of ethanol CC-2 of acetate D.C-2 of citrate E. C-1 of oxaloacetate F. none of the above.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liver homogenate under anaerobic conditions, which carbon in the lactate produced would be labeled with 1℃ (refer to the carboxyl carbon of lactate as C1, the central carbon as C2, and the methyl carbon as C3)? What if theglucose were labeled at carbon #3 instead? In both cases, explain your answer.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated...
If glucose labeled with 14C at C-2 were metabolized in the liver, the first radioactive pyruvate formed would be labeled in (pick from list given below) i) all three carbons ii) its carbonyl carbon iii) its carboxyl carbon iv) its methyl carbon Ali B) ii C) iii D) iv E) A and C
Q4. Compound A is labeled at the methyl carbon with 14C (*). Under fasting conditions, the 14C label ends up in compound C during steady-state metabolism in liver. COO- çoo- FO +H COO- H *CH3 a. What is the metabolic pathway involved? Write the reaction including all reactants and products, no structures required (1) b. Which carbons in compound C will become labeled, and to what percent? Briefly explain. (3) c. What is the physiological basis and the molecular mechanism...
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