Question

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Put the following steps to the Polymerase Chain Reaction in correc order 1st Tube is heated to about 95 der Tube is cooled to 70 degrees Tag Polymerase reads the pare [Choose Tag Polym Tag Polymerase reads the parent DNA and attaches the correct nucleotides 5th Choose Hydrogen bonds between the two strands durature Whicco Tube is heated to about 95 degree Celcius 6th Choose The process starts with a segment of the DNA of Interest being added to the tube Semi-conservative replication is completed and repeated 7th Choose A PCR cocktail made of excess nucleotides, PCR Primer and tag Polymerase are added to DNA 8th Choose PCR Primer attaches to parent DNA Tube is cooled to 10 degrees Celcius

Answer 1

The PCR has the following steps:

Preparation of the PCR mix is made in the following way:

1. The process starts with the segment of DNA of interest being added to the tube.

2. A PCR cocktail made of excess nucleotides, PCR primers and taq polymerase are added to the DNA.

The first step in PCR is the denaturation where the DNA strand are separated.

3. The tube is heated to 95 degrees Celsius.

4. Hydrogen bonds between the two strand of DNA denature.

Annealing of the DNA happens with primers

5. Tube is cooled to 70 degrees Celsius.

6. PCR primers attach to the parent DNA.

Extension happens and the DNA is replicated.

7. Taq polymerase reads the parent DNA and attaches the correct nucleotides.

8. Semiconservative replication is completed and repeated.

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