Which of the following statements about Polymerase Chain Reactions is wrong? a. DNA is briefly heated...
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Which of the following statements about Polymerase Chain Reactions is wrong?
a. DNA is briefly heated to break the hydrogen bonds holding two strands together.
b. Primers hybridize to sequences at opposite ends of the target sequence on the same strand.
c. DNA is cooled to allow primers to form hydrogen bonds with the ends of the target sequence.
d. The three steps of PCR are denaturation, annealing, and extension.
e. PCR produces specific DNA fragments for cloning.
f. Taq polymerase adds nucleotides to the 3’ end of each primer.
Homework Answers
b.) Primers hybridize to sequence at opposite ends of the target sequence on the same strand.
Which is wrong. Because the one primer (Reverse primer) attaches to the top strand or 5' -3' of DNA while the second primer (Forward primer) attaches to the bottom strand or 3' -5' of the DNA. And these primers work simultaneously during the annealing process.
Other than this all the points described are true about PCR technique.
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THANK YOU!!
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