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1. Where are DNase I hypersensitivity sites found, and what do they indicate about the nature...

1. Where are DNase I hypersensitivity sites found, and what do they indicate about
the nature of chromatin?
2. What is a response element? How do response elements bring about the
coordinated expression of eukaryotic genes?
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Answer #1

1)

DNase I hypersensitivity sites found near the regulatory promoter elements. It is around 1000 nucleotides upstream of start site. The sensitivity to DNase 1 suggests a more open chromatin, allowing access of transcription factors. In this DNA is essentially more exposed.

2)

Most of the eukaryotic cells always do not possess operons and many of the eukaryotic genes may get activated by the same stimulus. The activation may result due to the presence of clustered operons which also possess the same promoter. So, the genes which are able to express themselves in cordination possess the capability yo respond to the same stimulus because of the presence of short regulatory sequences. Such sequences of DNA are known as response elements.

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