If you have a culture of E. coli at a concentration of 5x10bacteria/mL and you infect...

Question

Question

science biology

Answer 1

Answer #1

The concentration of phage should be 1.5 X 10⁸.

The multiplication of infection can be calculated as

MOI = Concentration of plaques forming phage ÷ Total number of host cells

Putting the values, we have

3 = Concentration of phage ÷ 5 X 10⁷

Or, concentration of phage = 3 X 5 X 10⁷ = 15 X 10^7.

Answer 2

Similar Homework Help Questions

You have a liquid culture of E. coli at 7.5 x 10^9 org/mL concentration. Draw a...

You have a liquid culture of E. coli at 7.5 x 10^9 org/mL concentration. Draw a serial dilution scheme to give a final concentration of 1.5 x 10^3 org/mL and indicate the volume of sample, volume of diluent, DF, TDF, and the concentration of each tube. Please explain steps. I am not too sure how to even start problems like these. Thank you!
5 points. Can a wild-type lambda phage infect and kill an E. coli host that harbors...

5 points. Can a wild-type lambda phage infect and kill an E. coli host that harbors a wild-type lambda lysogen? Yes or No? 5 points. Can a wild-type lambda phage infect and kill an E. coli host that does not make the LamB protein? Yes or No?
You want to dilute and plate an E. coli culture that has 3 *10 8 cells/ml....

You want to dilute and plate an E. coli culture that has 3 *10 8 cells/ml. If you needed to dilute the above culture, starting from the undiluted stock, to a concentration of 500 cells/ml, how could you perform a serial dilution to achieve the desired final concentration?

you are given three stock cultures- E coli at a concentration of 1.5 x 10^3 cfu/ml,...

you are given three stock cultures- E coli at a concentration of 1.5 x 10^3 cfu/ml, Bacillus at a concentration of 4.2 x10^3 cfu/ml, and staphylococcus at a concentratino of 2.4 x10^4 cful/ml. you ahve been asked to make a 30 ml of a single mixed culture with a final concentration of 500 cfu/ml of E coli, 150 cfu/ml of bacillus, and 80 cfu/ml of Staph. How would you make this mixture?
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...

1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
You have a stock of an E. coli cell culture of an unknown concentration. You want...

You have a stock of an E. coli cell culture of an unknown concentration. You want to set up a serial dilution experiment so that you can count the viable colonies and determine the concentration of the stock in cells/ml. In tube A, you add 1 ml of the stock to 999 ml of media. In tube B, you add 1 ml of the material from tube A to 24 ml of media. In tube C, you add 50 microliters...

3. (4) Suppose that you use a phage P1 grown on a strain of E. coli...

3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
A culture of E. coli is diluted 1:1000. 1 ml of the 1:1000 dilution is spread...

A culture of E. coli is diluted 1:1000. 1 ml of the 1:1000 dilution is spread onto an agar plate. After incubation, 50 colonies are observed. What is the concentration of the original culture? Show your work and include proper units for full credit.
　 3. You inoculate 1 liter of medium with an E. coli culture to an initial cell...

　 3. You inoculate 1 liter of medium with an E. coli culture to an initial cell density of 104 cells/ml. The culture grows exponentially with a generation time of 20 minutes. What is the culture density after 24 hours?

Suppose your professor handed you a test tube with 2.0 ml of an E. Coli broth culture in it and...

Suppose your professor handed you a test tube with 2.0 ml of an E. Coli broth culture in it and told you to make a 10 -1 dilution of the entire culture. Explain howyou would do this. Show your calculations.