Question

Crispr/Cas9-based genome editing can be used to:

Question options:

	a). Create small deletions within genes
	b) Generate linkage disequilibrium
	c) Create gain-of-function mutations in genes
	d) a) and c)
	e) suppress crossing over in a genomic region

Answer 1

Answer: d. a and c

Crispr/Cas9 can be used to

                             Create small deletions within genes

                             Create gain-of-function mutations in genes

CRISPR/Cas9 is the most modern genome editing tool adopted for deletion, addition, and alteration of DNA. Cas9 is a molecular scissor protein used to create site-specific cuts in DNA sequences. A piece of RNA called guide RNA will lead the Cas9 nuclease into the specific location of DNA. The double-strand cuts created by Cas9 will allow any deletion or addition of new DNA fragments.

CRISPR has nothing to do with cross over and recombination.