Homework Answers
- Mutation in Rb gene prevents the production of tumor suppressor protein of the gene product called pRb.
- The non-phosphorylated form of pRb prevent progression of cell cycle beyond S-phase.
- Without pRb uncontrolled cell growth results in tumor formation.
- Upon phosphorylation, the transcription factor E2F is released from RB and help in transcription. This results in transition of cell cycle from G1 to S phase.
- Upon mutation, E2F is constitutively released, resulting in uncontrolled cell division.
- RB-E2F association is mediated though phosphorylation of several amino acids (like alanine).
- If there is mutation in the phosphorylation sites, this leads to conformational change and release of E2F.
Answer: Amino acids surrounding its phosphorylation sites.
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Question 36 27 Where would you predict mutations in E2F most commonly occur at? O its...
Match the following phenotypes with the mutation that would be the most likely to be its...
Match the following phenotypes with the mutation that would be the most likely to be its cause. The mutant proteins are listed below, followed by the protein domain that is mutated in each case, followed by the amino acid change that is a result of the mutation Try to answer each question by using the following reasoning: if you observe a phenotype, then that could be due to a mutation in protein in which the domain has had an amino...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
1. You have identified a new protein and predict it is a transmembrane protein due to...
1. You have identified a new protein and predict it is a transmembrane protein due to its AA sequence analysis. How would determine where the protein is located in the cell? A. Extract all the proteins from the cell and analyze them on a protein gel. B.Use Polymerase Chain Reaction (PCR) to amplify the protein. C. Use an fluorescent antibody to the protein and allow it to bind to the cells. D Isolate the RNA for the protein and use...
Explain why its the answer 1. Which amino acid sequence would most likely be for the...
Explain why its the answer 1. Which amino acid sequence would most likely be for the transmembrane domain of a plasma membrane protein a) SKDTRENHQY b) KRHGPRKHRK C) DEWFDEESDT D)SFNQYCGQCN E) GAVMVAIGIL 36. What is the typical progression of steps in PCR reaction a) annealing of forward and reverse primers, denaturation of a DNA template, extension by a DNA polymerase using deoxyribonucleotides b) denaturation of a DNA template , annealing of forward and reverse primers, extension by a DNA polymerase...
Question 7 2 pts Which of these genes would not likely be regulated by the bacterial...
Question 7 2 pts Which of these genes would not likely be regulated by the bacterial SOS response?! Translesion DNA polymerase Cell division promoter Holliday junction branch migration enzyme Nucleotide excision repair enzyme Question 8 2 pts Which of these mutations is likely to have the greatest impact on the amino acid composition of the resulting protein? Silent Synonymous Frameshift Missense Question 9 2 pts What would be the result of perfect and continuous suppression of the lac operon in...
QUESTION 13 Which of the following would occur if a mutation caused Kinase 1 to be...
QUESTION 13
Which of the following would occur if a mutation caused Kinase 1
to be unable to be phosphorylated? (Select all)
RTK would bind VEGF
RTK would phosphorylate itself
RAS would become active
The phosphorylation cascade would occur
The endothelial cell would divide
0.2 points
QUESTION 14
Imagine that an endothelial cell has a mutation in several of
the enzymes that perform mismatch repair. The endothelial cell
replicates its DNA and then divides into two cells. The resulting...
please answer all 6 questions Question 27 3 pts TRBP is a protein important for the...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
Question 1 Match the term with the best definition or description; most topics relate to the...
Question 1 Match the term with the best definition or description; most topics relate to the regulation of gene expression. General type of protein which will increase transcription rates when it attaches to a site A. Factor connected to a particular gene - B. Co-repressor C. Enhancer D. Promoter E. Structural F. Intron G. Activator H. Operator I. Basal transcription J. Glucocorticoid receptor K. Sigma factor L. Mediator M. Inducer N. TATA box O. Repressor The rates of mRNA produced...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
30. Harmful mutations in the mitochondrial genes coding for the electroe transport system would most atfect which of the processes? Choose the best answer A. oxygen diffusion ito red blood cells...
30. Harmful mutations in the mitochondrial genes coding for the electroe transport system would most atfect which of the processes? Choose the best answer A. oxygen diffusion ito red blood cells B. urine flow out of the urethra C. rapid beating of a hummingbirds wings D. formation of the phospholipid bilayer from free phospholipids E. the Calvin cycle CSLO6: AN 31. A specific poison blocks H' transport across the inner membrane of the mitochondria How will this directly affect the...
Match the following phenotypes with the mutation that would be the most likely to be its cause. The mutant proteins are listed below, followed by the protein domain that is mutated in each case, followed by the amino acid change that is a result of the mutation Try to answer each question by using the following reasoning: if you observe a phenotype, then that could be due to a mutation in protein in which the domain has had an amino...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
Question 7 2 pts Which of these genes would not likely be regulated by the bacterial SOS response?! Translesion DNA polymerase Cell division promoter Holliday junction branch migration enzyme Nucleotide excision repair enzyme Question 8 2 pts Which of these mutations is likely to have the greatest impact on the amino acid composition of the resulting protein? Silent Synonymous Frameshift Missense Question 9 2 pts What would be the result of perfect and continuous suppression of the lac operon in...
QUESTION 13
Which of the following would occur if a mutation caused Kinase 1
to be unable to be phosphorylated? (Select all)
RTK would bind VEGF
RTK would phosphorylate itself
RAS would become active
The phosphorylation cascade would occur
The endothelial cell would divide
0.2 points
QUESTION 14
Imagine that an endothelial cell has a mutation in several of
the enzymes that perform mismatch repair. The endothelial cell
replicates its DNA and then divides into two cells. The resulting...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
Question 1 Match the term with the best definition or description; most topics relate to the regulation of gene expression. General type of protein which will increase transcription rates when it attaches to a site A. Factor connected to a particular gene - B. Co-repressor C. Enhancer D. Promoter E. Structural F. Intron G. Activator H. Operator I. Basal transcription J. Glucocorticoid receptor K. Sigma factor L. Mediator M. Inducer N. TATA box O. Repressor The rates of mRNA produced...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
30. Harmful mutations in the mitochondrial genes coding for the electroe transport system would most atfect which of the processes? Choose the best answer A. oxygen diffusion ito red blood cells B. urine flow out of the urethra C. rapid beating of a hummingbirds wings D. formation of the phospholipid bilayer from free phospholipids E. the Calvin cycle CSLO6: AN 31. A specific poison blocks H' transport across the inner membrane of the mitochondria How will this directly affect the...
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