Question

Solution Preparation Calculations There are three solutions that need to be made. Be sure to have the calculations for all three of these solutions. Since these volumes are enough for the entire lab, I will assign lab groups to make different solutions (but calculations for all solutions need to be in your lab notebook). On the "Observations" side of the protocol, state who is preparing the solution. Solution 1 (Buffer) 0.1 M Sodium phosphate buffer, pH 6.8. Prepare 500 ml. NaH PO-H2O MW 137.99 Na:HPO-7H.O MW 268.08 Solution 2 (Substrate): 4 mg/ml DL-DOPA Prepare 150 ml Solution 3 (Inhibitor): 1x 10M phenyl thiourea (MW- 152.2) in ethanol. Prepare 25 ml. (Hint: you'll have to make a concentrated stock solution first.) Crude Potato Extract (Enzyme): Be sure to still list this part of the procedure in your lab notebook even if another group is preparing the extract. On the "Observations" side of the protocol, state who is preparing the extract. Procedure

Answer 1

1. for 1 L 137 + 268 g of salt

for 500 ml add 69 + 134 g of salt for 1M

for .1 M add 6.9 + 13.4 g salt and make up to 500 ml.