Homework Answers
The outcome that needs to be minimized is C) off site targeting as it might result in incorporation of replacement DNA into wrong place that may or may not be crucial for the survival of the organism and also there might be no change in phenotype due to presence of defective DNA.
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Which of the following outcomes do you want to minimize during genome editing? A. removal of...
1. Pre-messenger RNA processing d. What is RNA editing? Are you prepared to identify how a...
1. Pre-messenger RNA processing d. What is RNA editing? Are you prepared to identify how a single site of editing could change the expression of an mRNA? e. What are the catalytic sites within an intron? How are they recognized by the spliceosome (you do not have to memorize exact interactions, just understand generally how this occurs)? f. What is the mechanism of catalysis carried out by the spliceosome? How is this similar to Group I and Group II introns?...
Which of the following viruses are commonly used for gene delivery during genome editing? A. Adenovirus...
Which of the following viruses are commonly used for gene delivery during genome editing? A. Adenovirus B. measles virus C. Influenza D. rhinovirus
25. Which of the following is not true concerning eukaryotic mRNA processing (A) addition of a...
25. Which of the following is not true concerning eukaryotic mRNA processing (A) addition of a 3' poly-A tail (D) occurs in the nucleus (C) intron splicing (B) addition of a S' cap (E) occurs in the cytoplasm 26. Which of the following is involved in maintaining lysogeny in (A) PRE (B) PRM (C) PRI (D) PRO (E) all of the choices are correct Use the following diagram to answer questions 27-28. Each circle represents a DNA molecule and the...
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the...
Which of the following statements is FALSE? In the context of CRISPR-Cas9 gene editing system, the PAM sequence: a. A sequence adjacent to the target DNA sequence to be cleaved b. Is present on the non-complementary target DNA strand to the crRNA c. Has the sequence “5’-NGG-3’” where N is any nucleotide d. Stands for "protospacer adjacent motif" e. Is present in the bacterial genome
Which of the following is NOT a feature of RNA processing in eukaryotes? a. Caps and...
Which of the following is NOT a feature of RNA processing in eukaryotes? a. Caps and tails are added to RNA transcripts before they leave the nucleus b. Multiple genes are represented in one mRNA from an operon c. Introns are removed and exons are spliced back together to make mature mRNA d . "Alternative splicing" means that one gene in the genome can encode multiple products e. The sequence that codes for one eukaryotic gene is not a continuous...
QUESTION 1 Which of the following statements regarding splicing is FALSE? A) The length...
QUESTION 1 Which of the following statements regarding splicing is FALSE? A) The length of introns determines the efficiency of splicing. B) Many human neurological disorders are caused by splicing errors and/or mutations in splicing factors. C) Splicing requires the action of a variety of snRNAs that direct the transesterification reactions. D) Splicing is dictated by sequence features in pre-mRNA transcripts QUESTION 2 Which of the following mRNA processing factors does NOT associate with...
1. Which of the following is more error prone? A) human genome replication b) RNA viral...
1. Which of the following is more error prone? A) human genome replication b) RNA viral genome replication c) DNA viral genome replication d) Canine genome replication e) the genetic diversity of virus 2. Which is Not a level of control for viral gene expression? A) Configuration of viral DNA or RNA b) At transcription c) at regeneration d) mRNA half-life e) at translation 3) which of the following defines the Baltimore scheme? A) a chart of DNA transcription mechanisms...
Which of the following is a correct statement? Select one: a. If you want to make...
Which of the following is a correct statement? Select one: a. If you want to make a cDNA library, it doesn't matter which tissue you isolate the mRNA from. b. If you want to make a genomic library, it doesn't matter which tissue you isolate the DNA from. c. If you want to make a cDNA library, it doesn't matter which tissue you isolate the DNA from. d. If you want to make a genomic library, it doesn't matter which...
You want to know which areas of the genome are transcribed. Which of the following experiments...
You want to know which areas of the genome are transcribed. Which of the following experiments would give you the most complete answer of the research question? Make a genomic DNA library and sequence it Make a cDNA library and sequence it Hybridize a tilling array gene chip with the labeled RNA (or cDNA derived from RNA) extracted from the cell Analyze the proteins expressed by the cell using mass spectrometry
(7) Which of the following structures is type 1 cap attached to a eukaryotic mRNA? a....
(7) Which of the following structures is type 1 cap attached to a eukaryotic mRNA? a. m''ppp Sex b. m'G'ppp SXmY C. m’sppp XmYmZ d. None of the above (8). What are cryptic splice sites? a. These are splice sites that are used in some cells, but not in others b. These are splice sites that are always used. c. These are sites that are involved in alternative splicing, resulting in the removal of exons from some mRNA molecules d....
25. Which of the following is not true concerning eukaryotic mRNA processing (A) addition of a 3' poly-A tail (D) occurs in the nucleus (C) intron splicing (B) addition of a S' cap (E) occurs in the cytoplasm 26. Which of the following is involved in maintaining lysogeny in (A) PRE (B) PRM (C) PRI (D) PRO (E) all of the choices are correct Use the following diagram to answer questions 27-28. Each circle represents a DNA molecule and the...
(7) Which of the following structures is type 1 cap attached to a eukaryotic mRNA? a. m''ppp Sex b. m'G'ppp SXmY C. m’sppp XmYmZ d. None of the above (8). What are cryptic splice sites? a. These are splice sites that are used in some cells, but not in others b. These are splice sites that are always used. c. These are sites that are involved in alternative splicing, resulting in the removal of exons from some mRNA molecules d....
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