The post doctoral fellow asks you to prepare 500 ml of RPMI media to culture RAW...
The post doctoral fellow asks you to prepare 500 ml of RPMI media to culture RAW 264.7 cells. How do you complete media containing 5% FBS and antibiotics?
Homework Answers
The RAW 264.7 cells must be grown in DMEM (Dulbecco's Modified Eagle's medium) with 10%FBS (Fetal Bovine Serum) and antibiotic (generally penicillin 100U) and 1% L-glutamin. Growing on RPMI 1640 media also requires 10% heat-inactivated FBS.
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The post doctoral fellow asks you to prepare 500 ml of RPMI
media to culture RAW...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a...
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a series of 1 or 10 ml dilutions (maximum 1:100) to obtain a final 200 ml culture at 1x103 cells/ml? Show your work for each step
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a...
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a series of 1 or 10ml dilutions (maximum dilution 1:100) to obtain a final 200 ml culture at 1x103 cell/ml? Provide work for each step.
It would be much appreciated if you can help me with the recipe of the buffer...
It would be much appreciated if you can help me with the recipe
of the buffer above. Thank you.
Cells are kept in suspension in RPMI, a media for growing cells. You have 30 mL of a suspension at a concentration of 7.2x10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1*10^5 cells when plated in the wells of a 96-well microtitre plate. How would you dilute...
You received a T125 flask containing HMEC-1 cells in 25 ml of media. You need to...
You received a T125 flask containing HMEC-1 cells in 25 ml of media. You need to split and also freeze the cells. First you removed the old media and washed your cells with 10 ml of PBS. The PBS was removed and 20 mls of fresh media was added to the cells. The cells were scraped and re-suspended in the 20 mls of media. To count your cells you added 125 ul of cells to 375 ul of PBS. Then...
If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much...
If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much stock cuiture and how much media would you add to obtain a final concentration of 2.5*10 cells/ml in a final volume of 20mL? (1 point)
If you started with adding 50 ul of an overnight culture to 500 mls of LB...
If you started with adding 50 ul of an overnight culture to 500 mls of LB media and grew the culture at 37oC for 4 hours and plated 200 ul of a cell suspension and observed 100 colonies on your plate, how many cells were in the original overnight culture used to inoculate the LB media?
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
please help solve this and show all work clearly, thanks 6. The recipe for cell culture media is: 10 % serum, 1x ant...
please help solve this and show all work clearly, thanks
6. The recipe for cell culture media is: 10 % serum, 1x antibiotics and 2 mM Glutamine in basal media. You are given the following stock solutions: • Basal media • Serum 100% • Antibiotics 100x solution Glutamine 200 mm How will you prepare the 500 mls of media? (2 point) (Note: A 100X solution is 100 times more concentrated than the concentration required in the final solution. For example...
1) If you have a 5 molar solution and a diluted 0.1 molar solution. What is...
1) If you have a 5 molar solution and a diluted 0.1 molar solution. What is the ratio between these two? 2) If you want to prepare 50ml medium containing 5% FBS. What is the volume of FBS you have to add? 3) If the concentration in your original medium is 50.000 cells per ml and you add 1ml of this medium to 4ml fresh medium without cells. How many cells per ml are in these 5ml of new medium?...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
It would be much appreciated if you can help me with the recipe
of the buffer above. Thank you.
Cells are kept in suspension in RPMI, a media for growing cells. You have 30 mL of a suspension at a concentration of 7.2x10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1*10^5 cells when plated in the wells of a 96-well microtitre plate. How would you dilute...
If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much stock cuiture and how much media would you add to obtain a final concentration of 2.5*10 cells/ml in a final volume of 20mL? (1 point)
please help solve this and show all work clearly, thanks
6. The recipe for cell culture media is: 10 % serum, 1x antibiotics and 2 mM Glutamine in basal media. You are given the following stock solutions: • Basal media • Serum 100% • Antibiotics 100x solution Glutamine 200 mm How will you prepare the 500 mls of media? (2 point) (Note: A 100X solution is 100 times more concentrated than the concentration required in the final solution. For example...
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