Question

answers are given in bold. im not understanding how they got the answer so can you please show me and or explain to me how they got it as simple as possible. tips and tricks in what to look for when answering these questions. will rate only if All questions are answered (28 through 36) please

28-31. (4 points each, 16 points total) For questions 28-31, indicate which of the following patterns of expression would be observed in strains with the indicated genotype. The'+' marks indicate when the gene produces an enzyme, a no enzyme produced. The lac mutation has defective promoter sequences that indicates prevent RNA polymerase from binding. Without lactose lacz lacY With lactose lacz lacY cue + + + Question Genotype of partial diploid E. coli strain 1 P+ 0 Z+ Y/ I* P+ 0* Z-Y+ P+ 0* Z Y / P O Z Y+ I P* O Z* Y I P+ 0+ Ꮓ Y+ IP-O* Z* Y+ /1 P+ 0 Z+ Y 32-36. (2 points, 10 points total) Answer Aifa statement is true and B if it is false. 32_F_The lariat structure formed during intron splicing stabilizes the mRNA. 33_F_The exon junction complex holds the two exons together after splicing. 34_T_Alternative splicing allows many different proteins to be produced from a single gene. 35_F_Missense mutations cause translation to terminate too soon. 36_T_The promoters of both prokaryotic and eukaryotic genes often include sequences composed mostly of As and Ts that help determine where transcription starts.

Answer 1

Lac ZYA Lac Is Repressor Z anon Frat B-Galactosidase I -> Repriser ptn not bind 'operates constilubie y B-Galach sidose 4 pesmede always Trays whether Lac Pregata SB-halactos PA I JP10ŹYA not Result I PROIZYA) 12 YA Represen Pin has a modified Lac Bindins site J. Besn't allow Lac molecule To Bind. No once Represim bound to 'Operator D i t not remore even it Lac available in na sad time Lac here not Transcribe. oss operatur Site modified So Repres mis net Bind Ey Gaylitulie. P B Galadonide a permesse always travenbed B Gal Production whether Lac Present Lal Prese TLAL Abset M or not. ROVA PSImbrose Brd here pe Permeye made + 1 + It pt of at yt I pt of at yt qS pt of zT yT It A of 3+ yt It pt of it yt It pt st 2 yt it otot stý titit I Į ti 1 tt

23). I pt oC zty 2. It prof zyt Hi a n Prepressive It binds it of not oC so 24 Ach as Indröble spenn bw yt 1 y Expres cwnglitulve operon ont presere st ruche for at lac z expres 29) A IS.pt of zty with a wisat lactose IS s Binck to It pt of Eys ot and not releve eiver addition of Activator Lactose I s Suppleve mutation ocors at lactove binding site. So Tachose not able to bind with Is so awas operon Stop BOBI I Bol pt of z y mistated Represr not able to pt of zy bind operator resion. Constitive expres. zt, yt alass express cron,

was exprea roN. 3) DEPO 2 7 p ot at yty i pt of ztý promoter mutated so blo vse of this opewn. So don't Corsider this opern. O s operator mutated. No repressor binds here. oss operator mutate go 2t - Always express y s so lacy Not-expren at all. 32. Zariat strucve is led to cut te inton it not have any de in stable the mRNA. 33 F 357 Allernate Sphins makes different protein from single sore. 35 F ong Nonsense motation terminate translation po doon. 38 Nost clear visible.