Question

An enzyme catalyzes the reaction A ® B. The initial rate of the reaction was measured as a function of the concentration of A (substrate). The following data were obtained: [10 pts.]

[A], micromolar         V₀, nmoles/min

                  0.05                            0.08

                  0.1                              0.16

                  0.5                              0.79

                  1                                  1.6

                  5                                  7.3

               10                               13

               50                               40

             100                               53

             500                               73

         1,000                               76

         5,000                               79

       10,000                               80

       20,000                               80

Answer the following questions:

What is the K_m of the enzyme for the substrate A?

What is the value of V₀ when [A] = 43?

What is the value of the y-intercept of the line?

What is the value of the x-intercept of the line?

Hint: Plot 1/ V₀ vs. 1/[A], and a straight line will be obtained (for c and d).

Please attached a copy of your graph for a full point for this question (in excel format)

Answer 1

ne weawe Bonh plot km na t man Km 0

20 12.5 10 6.25 1.27 1 0.62 0.2 0.137 0.1 0.077 0.02 0.025 0.01 0.019 0.002 0.014 0.001 0.013 0.0002 . 0.0126 0.0001 0.0125 0.00005 0.0125 14 y 0.624x+0.011/ 2 12 10 4 Seriesl Linear (Seriesi) 0 0 10 15 20 25

n Slopo Tnencbh m Q63u km 56.2久 丙201023 0 voz 39-시 0.011