A scientist is doing a classical Sanger sequencing reaction. He sets up four reaction tubes, labeling them G, A, T, and C. To each tube he adds template, DNA polymerase, buffer, primer, all four dNTPs, and accidently, small amounts of all four ddNTPs. He then runs his samples on a sequencing gel. What do you think the gel will look like?
The G, A, T, and C lanes will all look like normal, and it will still be easy to read the sequence.
The G, A, T, and C lanes will show an identically sized band that is exactly the same size as the unextended primer.
The G, A, T, and C lanes will show an identically sized band that is exactly one base longer than the unextended primer.
All four lanes will show identical patterns that look like ladders of many fragments, each of which is one-base different in length.
Answer
The G, A, T, and C lanes will show an identically sized band that is exactly one base longer than the unextended primer that one base will dideoxy base.
A scientist is doing a classical Sanger sequencing reaction. He sets up four reaction tubes, labeling...
1) You have four tubes with buffer, primer, DNA polymerase, template, and dNTP’s. In tube A, you have added radioactive ddATP at a ratio of 1 ddATP/100 dATP. In tube T, you have added radioactive ddTTP at a ratio of 1 ddTTP/100 dTTP. In tube G, you have added radioactive ddGTP at a ratio of 1 ddGTP/100 dGTP. In tube C, you have added radioactive ddCTP at a ratio of 1 ddCTP/100 dCTP. Here is a picture of the template...