1. 1000 micro litre = 1mL
Or, 20 micro L = (1/1000)*20 = 0.02 mL
2. The charge on DNA molecule is Negative i.e., DNA is negatively charged .The negative charge is due to presence of the phosphoric acid in the DNA backbone.
3. The gel has pores of uniform specific size .If the DNA sample has size more , then it travels slowly via the pores and hence do not go farther than the gel but if the DNA sample is small, it passes easily through the pores and hence can go farther from the gel. So, less the size, the more farther it goes and vice versa.
4. Before putting the DNA sample into the wells, they are mixed with a dye of a specific colour ( orange, blue etc). After electrophoresis when the gel is seen under UV light, the Band fluoresces due to the dye mixed earlier and this is how we can visualise the DNA sample.
is a nucleus the sole source of DNA in a eukaryotic cell? WORKSHEET: EXERCISE 10 NAME:...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
help with questions 5 to 10 please PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2 marked by the yellow arrow. Thanks! Here is the only other info I have. Thanks! Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It is called the SMALL gene. i atgggattac actgtcacga ccaaatagcc ttcattgtat 41 caaaaggato aatcgagtta tag Imagine you are doing a research project in a laboratory and your supervisor asks you to clone the SMALL gene into the PBR322 plasmid (shown below). You must use the Pstl and EcoRI sites for your cloning. HindIII EcoRI | EcoRV BamHI 4359 0 29 185 4000 375 Sall Psti...
These are previous exam questions I am just preparing for the final exam by studying the previous questions! please help me out! A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It is called the SMALL gene. 1 atgggattac actgtcacga ccaaatagcc ttcattgtat 41 caaaaggatc aatcgagtta tag Imagine you are doing a research project in a laboratory and your supervisor asks you to clone the SMALL gene into the pBR322 plasmid (shown below) You must...
For part 1 of this lab) I collected a soil sample from my campus Part 2) Tested bacteria initial viability Part 3) DNA extraction Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA sequence data analysis (sent sample to another lab) Directions for Part 3 DNA extraction are in the attached image QUESTIONS REGARDING PART 3 (DNA extraction) 1) What type of conclusions can be made from initially...
help on number 3 here is lab we did EXPERIMENT 19 An Analysis of Commercial Antacids Rolaids Alka Seltzer OBJECTIVES: Determine the per gram neutralizing ability of a commercial antacio Determine the per gram neutralizing ability of sodium bicarbonate Compare the effectiveness of several antacids. INTRODUCTION: "How do you spell relief two hecimie The parietal cells in the stomach secrete sufficient hydrochloric aci 0.10 M The flow of HCl increases when food enters the stomach and so acid secrete sufficient...