5) You perform 4 DNAsel footprinting experiments. All the experiments contain DNA 1. Epxeriments 2, 3, and 4 also contain Protein A which binds DNA 1 in the indicated position. DNA 1 is labeled by radio lables on the indicated ends. Arrows show positions that DNAsel can cut. Draw the banding pattern you epect to see in the 4 different experiments in the box on the right.
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5) You perform 4 DNAsel footprinting experiments. All the
experiments contain DNA 1. Epxeriments 2, 3,...
Review. Consider how one might label the 5'ends of DNA for a footprinting experiment where you...
Review. Consider how one might label the 5'ends of DNA for a footprinting experiment where you visualize where on the DNA a transacting protein binds to the cis acting promoter sequence The 5 phosphate(s) at the S'ends a DNA can be removed using a phosphatase, like calf intestinal phosphatase (CIP), in vitro, leaving a 5-hydroxyl group at the end of DNA CIP has no impact on the phosphates along the backbone of the DNA, just the 5' end phosphate and...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is drawn incorrectly, they should be the same One is o pure onsa pyrimidine. c. One is a purine, one is a pyrimidine d. Double-stranded DNA is antiparallel. e. This is after DNA replication -before DNA replication, they look the same. 3....
You are a scientist working for a lab attempting to develop an anti-obesity drug called glucono....
You are a scientist working for a lab attempting to develop an anti-obesity drug called glucono. The idea is that glucono could be added to the diet to reduce glucose and calorie absorption; even though glucono would be absorbed, it would not be metabolized, and would be excreted by the kidney. The hypothesis is that glucono binds to the Na+/glucose transporter that is a part of secondary active transport of glucose. This cannot be easily studied in humans, so you...
can someone explain how to answer this with reasons why 30-45 mins after estrogen addition: Acchyiase...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
Please I need help on questions 1-4 in great detail please Load 15 mu l of...
Please I need help on questions 1-4 in great detail please
Load 15 mu l of the following samples from the above section onto the simple Wells. Seal the wells with agarose and electrophorese until the bromophenol blue in the samples has migrated to within 2 mm of the positive electrode end of the gel. Remove the gels from the unit and stain them as described in Section IV. Measure the distance of the DNA bands (in cm) from the...
help! 1 , 2 and extra credit! please answer all. 1. You study the following reaction...
help! 1 , 2 and extra credit! please answer all.
1. You study the following reaction in a series of experiments: (CH),COH(aq) + HCl(aq) à (CH),CCl(aq) +H,0(1) At 100.C you obtained the following data: Experiment (CH.COH., MHCIL. M Initial Rate, M's 100 0.100 5.0 x 10 0.100 10 x 10 300 6.200 10x10 0.300 0.100 1.5 x 10 0.00 Please do the following: a. Write the rate law for the reaction. b. Indicate the order of the reaction with respect...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
Review. Consider how one might label the 5'ends of DNA for a footprinting experiment where you visualize where on the DNA a transacting protein binds to the cis acting promoter sequence The 5 phosphate(s) at the S'ends a DNA can be removed using a phosphatase, like calf intestinal phosphatase (CIP), in vitro, leaving a 5-hydroxyl group at the end of DNA CIP has no impact on the phosphates along the backbone of the DNA, just the 5' end phosphate and...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is drawn incorrectly, they should be the same One is o pure onsa pyrimidine. c. One is a purine, one is a pyrimidine d. Double-stranded DNA is antiparallel. e. This is after DNA replication -before DNA replication, they look the same. 3....
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
Please I need help on questions 1-4 in great detail please
Load 15 mu l of the following samples from the above section onto the simple Wells. Seal the wells with agarose and electrophorese until the bromophenol blue in the samples has migrated to within 2 mm of the positive electrode end of the gel. Remove the gels from the unit and stain them as described in Section IV. Measure the distance of the DNA bands (in cm) from the...
help! 1 , 2 and extra credit! please answer all.
1. You study the following reaction in a series of experiments: (CH),COH(aq) + HCl(aq) à (CH),CCl(aq) +H,0(1) At 100.C you obtained the following data: Experiment (CH.COH., MHCIL. M Initial Rate, M's 100 0.100 5.0 x 10 0.100 10 x 10 300 6.200 10x10 0.300 0.100 1.5 x 10 0.00 Please do the following: a. Write the rate law for the reaction. b. Indicate the order of the reaction with respect...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
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