Question

DQuestion 7 3 pts In your molecular biology class at Xavier Institute for Higher Learning you explore the mechanisms of DNA replication; happen if your fellow students lacked different components of the replisome (all the different enzymes that assemble at the replication bubble to help replication occur). What would occur if DNA polymerase llretained all its functions except 3-5' exonuclease activity? by imagining what would O The new DNA strand would grow more slowly (rate of daughter strand elongation would The new DNA strand would not grow at all the enzyme would no longer function o The new DNA strand would grow more quickly (rate of daughter strand elorngation would increase)

Answer 1

DNA polymerase III is a main replicating enzyme because of its high polymerization (1000 nucleotides per seconds) and processivity rate. It has four components- catalytic core component, dimerization componenet, processivity component and a clamp loader. Catalytic component consists of three subunits 5'-3' polymerase activity, 3'-5' exonuclease activity for proofreading and the $\Theta$ which enhace the proofreading activity. The 3'-5' exonuclease activity is responsible for proofreading which enhance the accuracy of the DNA synthesis. So, if the DNA polymerase III lacks the exonuclease activity the rate of mutation will increase. The overall replication rate will also increase.

Hence, answer C is right- The new DNA strand would grow more quickly (rate of daughter strand elongation rate will increase).