Question

Activity Form Procedure Activity Setup and follow the detailed procedure steps found under the Procedure tab Stepfanie Malone Species Case Data Run Modl Power Acme Power Inc 3 A C 2 Samples Electrophoresis Load Samples T07/4/2019 9:30 PM TEM Dim Lights Add Top Time: 30,0s Rese Data Run Completel Start Data Run Table Top Science Copyright 2016 2019 All Rights Reserved AUCRIOSCUt-electrolhorabs Tb eeoczip amava IMG 1691pg

1. Determine the DNA sequence by analyzing the location of each nucleotide across the four lanes of the electrophoresis instrument. Provide the DNA sequence as your answer here.

2. A biologist at World University used Procedure IV to conduct an additional study and found the sequence following below. Using your knowledge of central dogma generate the amino acid sequence that results from this sequence. Given DNA sequence: GTA CTC CAC GGA TGA

Answer 1

1) The gel contains the sequence of newly synthesised strand of DNA. In this gel, the 5' end is found at bottom of gel where as 3' end is found at top of gel because in gel the shorter fragments run faster and larger fragment run slower. The synthesis of DNA occurs from 5' end.

DNA sequence in gel:

5' TCAGCGTCACTAACGT 3'

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3)  

DNA :

5' GTA CTC CAC GGA TGA 3'

The mRNA synthesised from DNA will have following sequence.

5' GUA CUC CAC GGA UGA 3'

The amino acid is found from the mRNA codon table :

N val - leu - his- gly C