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2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to in this case This is defined as Vmax d) Rewrite equation I using Vmax instead so your expression should now look like rate-Vmax[S(Km+[S]) e) A Lineweaver Burk plot is a graph of I/rate (y-axis) vs. 1/[S] (x-axis). Based on the equation from part d), show what the slope and intercept of a Lineweaver-Burk plot will be. 4. A possible mechanism for the decomposition of ozone in the reaction 203 (g) → 3。2(g) is: ana uie dennst end with a) The intermediate in this reaction is

how we can solve q2

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K2 dt tate approrimation to led CLES rearranging EJ] Dmde NuMechawisw 2 Sulbetrate e* Ji ue unite rati sqM tate at んexin ation to Es alco, we know [El - DELES] Le iitial total contentra(dNo Maximum rate Vmax) wi occur whun all the initial concentration op entyme Yeac with Subd trato no enzqnu LL 3) we For uv

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