Scientists have genetically engineered a new DNA polymerase named Phusion polymerase. What is Phusion polymerase and...
Scientists have genetically engineered a new DNA polymerase named Phusion polymerase. What is Phusion polymerase and what properties does it have in comparison to Taq polymerase? (Hint: an excellent answer will make reference to parts of the E. coli replisome). (4 marks)
Homework Answers
when pyrococcus like enzyme is fused with the processivity enhancing domain results in increase in fiedility and speed .phusion polymerase is an ideal choice beacuse it can be used for difficult amplicons.its error rate is also lower than the taq ploymerase. it possess 5'to 3'polymerase activity and 3'to 5'exonuclease activity and also thermostabe.
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Scientists have genetically engineered a new DNA
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Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Scientists at the University
of Iowa are working on a live, genetically COVID-19 vaccine. They
have inserted the genes that encode COVID-19 envelop protein a
virus (parainfluenza). In the diagram, 1) viral vector vaccine.
Parainfluenza virus causes mild influenza-like
symptoms.
Diagram or explain the steps that were taken to get the
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