－How can Chimeric proteins be used to study protein sorting? － What effect would the addition...
－How can Chimeric proteins be used to study protein sorting?
－ What effect would the addition of a lysosome‐targeting signal have on the subcellular localization of
a. A protein that is normally cytosolic?
b. A protein that is normally secreted?
Homework Answers
How can Chimeric proteins be used to study protein sorting?
Chimeric proteins are translated from the RNA which is comprised of exons of two or more different genes. When a chimeric protein has a Glutathione S-transferase (GST) tag or a hexa-his peptide e.t.c, they can be used for protein sorting by using affinity chromatography with glutathione immobilized to a solid support (glutathione is the substrate for GST) or nickel or cobalt resins (histidine residues binds to immobilized metal ions, nickel, cobalt).
What effect would the addition of a lysosome‐targeting signal have on the subcellular localization of
a. A protein that is normally cytosolic?
The normally cytosolic protein does not have a signal sequence and does not enter the endoplasmic reticulam. Hence, addition of a lysosome-targeting signal would have no effect.
b. A protein that is normally secreted?
The addition of lysosome-targeting signal would direct a normally secreted protein to lysosomes from the Golgi apparatus.
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－How can Chimeric proteins be used to study protein sorting?
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