What is the difference between mM, uM, and nM when doing PCR and what do they...
What is the difference between mM, uM, and nM when doing PCR
and what do they stand for (ex. uM same as micromolar?)
Homework Answers
Ans:- These are three different concentration units mM (milli Molar = 10-3 molars) means any substance for example "A" is present at a concentration of one thousand of a mole per litre.
uM (micro Molar = 10-6) means any substance for example "A" is present at a concentration of one-millionth of a mole per litre.
nM (nano Molar = 10-9) means any substance for example "A" is present at a concentration of one-billionth of a mole per litre.
Add Answer to:
What is the difference between mM, uM, and nM when doing PCR
and what do they...
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount...
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified. O In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In PCR, the temperatures vary more than in RT-PCR...
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR...
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR deletes DNA b. Polymerase Copying Repeats; PCR amplifies DNA c. Polymerase Copying Releats; PCR deletes DNA d. Polymerase Chain Reaction; PCR amplifies DNA 2) During gel electrophoresis, the DNA fragments are separated by ____ a. charge b. DNA fragments cannot be separated c. color d. size 3) Primers are a. double stranded DNA oligonucleotide (fragment) b. double stranded RNA oligonucleotide (fragment) c. single stranded...
what is the difference between wu wei and doing nothing?
what is the difference between wu wei and doing nothing?
1.) What is the concentration of a solution of PCR primers if the absorption of 260...
1.) What is the concentration of a solution of PCR primers if the absorption of 260 nm (A260) is 0.478 the path length is 1.0 cm and the extinction coefficient (absorptivity) is 185 mM^-1 cm ? a. 0.39 mN b. 3.9 x 10^-3 M c. 2.6 x 10^-3 mmol/L d. 2.6 mmol/L 2.) What is the concentration of a stock solution of PCR primers if the absorption of a diluted solution at 260 nm (A260) is 0.528, the path length...
check all that apply What are some differences between the PCR that occurs in the Sherlock...
check all that apply
What are some differences between the PCR that occurs in the Sherlock protocol and the PCR that occurs in RT-PCR test? O PCR in Sherlock protocol can amplify more than one gene at the same time O Dyes are added to RT-PCR PCR in RT-PCR is faster PCR in Sherlock protocol is isothermic (all the steps occur at the same temperature).
What is the wavelength (in nm) of light falling on double slits separated by 1.5 um...
What is the wavelength (in nm) of light falling on double slits separated by 1.5 um if the third-order maximum is at an angle of 600? You need to round your answer to the nearest integer, do not include unit.
3. What is PCR? Find a scientific article that uses PCR to illustrate your knowledge of...
3. What is PCR? Find a scientific article that uses PCR to illustrate your knowledge of a. what it is used for b the regents used c. what controls were used and why, d. what other application can it be used for different from that used in the paper, d. Explain the use of primers in PCR, including why two different primers must be used. e. What relationship must exist between the two primers used in PCR? f. Which nuclease...
I'm doing an induction motor assignment and trying to find out the difference between Ns and...
I'm doing an induction motor assignment and trying to find out the difference between Ns and N in the equation......N=(Ns)*(1-S), please describe the difference, and what word should I look for when trying to identify Ns or N in a problem's description.
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor 0.02 0.04 0.10 0.25 1.00 2.50 0.8 2.9 8.6 24 36 50 Plot the data for the kinetics of the enzyme (with and without the inhibitor) in a double reciprocal (Lineweaver-Burk) plot. Keep in mind that the x axis is 1/[S] and the y axis is 1/Vo. If you are using Excel you want to choose the (x,y) scatter plot (without a ne). You...
17. In a double slit experiment, the distance between the slits is 0.2 mm and the...
17. In a double slit experiment, the distance between the slits is 0.2 mm and the distance to the screen is 100 cm. What is the phase difference (in degrees) between the waves from the two slits arriving at a point 5 mm from the central maximum when the wavelength is 400 nm? (Convert your result so the angle is between 0 and 3600.) ANS-180°
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified. O In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In PCR, the temperatures vary more than in RT-PCR...
check all that apply
What are some differences between the PCR that occurs in the Sherlock protocol and the PCR that occurs in RT-PCR test? O PCR in Sherlock protocol can amplify more than one gene at the same time O Dyes are added to RT-PCR PCR in RT-PCR is faster PCR in Sherlock protocol is isothermic (all the steps occur at the same temperature).
What is the wavelength (in nm) of light falling on double slits separated by 1.5 um if the third-order maximum is at an angle of 600? You need to round your answer to the nearest integer, do not include unit.
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor 0.02 0.04 0.10 0.25 1.00 2.50 0.8 2.9 8.6 24 36 50 Plot the data for the kinetics of the enzyme (with and without the inhibitor) in a double reciprocal (Lineweaver-Burk) plot. Keep in mind that the x axis is 1/[S] and the y axis is 1/Vo. If you are using Excel you want to choose the (x,y) scatter plot (without a ne). You...
17. In a double slit experiment, the distance between the slits is 0.2 mm and the distance to the screen is 100 cm. What is the phase difference (in degrees) between the waves from the two slits arriving at a point 5 mm from the central maximum when the wavelength is 400 nm? (Convert your result so the angle is between 0 and 3600.) ANS-180°
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago