Why is there no LacA expression in the -Lactose column? SInce there is a mutation in lacI, shouldnt it be constantly on dispite lactose?
Homework Answers
This can be explained by the concept of cis and trans. Operator and promoter are the cis acting that is acting from the same molecule and the repressor(I) is trans acting that is the repressor can go and bind to far operator of other molecule also. Here there only one repressor is mutated, the repressor from the wild type(lacI+) can bind to the operator of the host.
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Why is there no LacA expression in the -Lactose column? SInce
there is a mutation in...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What is a possible genotype of the cells? Group of answer choices lacI+ lacP+ lacO+ lacZ+ lacY+ lacA+ lacl+ lacP+ lacO+ lacZ+ lacY+ lacA+ lacI- lacP+ lacO- lacZ+ lacY+ lacA- lacI+ lacP+ lacO+ lacZ- lacY- lacA+ lacI- lacP+ lacOc lacZ+ lacY+ lacA+
Microbiology question 51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for...
Microbiology question
51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
my teacher was useless with the method for doing these problems can someone please guide me...
my teacher was useless with the method for doing these
problems can someone please guide me in the right direction. dont
do all of them, but if you do at least explain how you came to that
conclusion. thank you
u
6. For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of B-galactosidase and permease and a minus sign (-) to indicate no synthesis of the proteins. Lactose absent Lactose...
For the two questions below, the genotypes indicated would lead to what type of expression of...
For the two questions below, the genotypes indicated would lead to what type of expression of βgalactosidase with respect to lactose inducibility: Is it inducible, constitutive or non-inducible. Explain in brief? F'lacI+ lacOc lacZ- lacY+ lacA+/ lacI+ lacO+ lacZ+ lacY+ lacA+ F'lacIs lacO+ lacZ+ lacY+ lacA+/ lacI+ lacO+ lacZ+ lacY+ lacA+
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions,...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
Imagine you are carrying out research on the lac operon. You isolate six mutations in the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
Microbiology question
51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
my teacher was useless with the method for doing these
problems can someone please guide me in the right direction. dont
do all of them, but if you do at least explain how you came to that
conclusion. thank you
u
6. For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of B-galactosidase and permease and a minus sign (-) to indicate no synthesis of the proteins. Lactose absent Lactose...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
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