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In a recent publication, gold nanoparticles conjugated to DNA(self-hybridized) exposed to Radio Frequency (RF) energy resulted...

In a recent publication, gold nanoparticles conjugated to DNA(self-hybridized) exposed to Radio Frequency (RF) energy resulted in the dehybridization of the DNA as shown in the figures below:

Image result for In a recent publication, gold nanoparticles conjugated to DNA(self-hybridized) exposed to Radio Frequency (RF) energy resulted in the dehybridization of the DNA as shown in the figures below:

A. Describe the potential structure of the hybridized and dehybridized DNA.

B. Describe how the A260 reading could change under RF.

C. Propose a mathematic equation to model the dehybridization seen in the figure.

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Answer #1

A. Secondary structure not really favours hybridization. Instead dehybridization is favoured by it. Hybridization in the other hand is favoured by the primary structure. Regarding absorption, single stranded helix are much more prone to get absorbed on the gold nanoparticles.

B. A260/A280 ratio tells about the purity of the DNA molecule examined. Thus radio frequency ablation will defenitely affect this ratio which is strictly a purity measure.

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