A patient has a CH50 result of 2%. can you determine which complement protein/proteins are deficient...
A patient has a CH50 result of 2%. can you determine which complement protein/proteins are deficient using this assay?
Homework Answers
There are around 30 blood protiens which are deficient in CH50 test...they are lymphocytes,cytokines,completement proteins, phagocytes,serum albumin,globulin,fibrinogen etc.
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A patient has a CH50 result of 2%. can you determine which
complement protein/proteins are deficient...
A graduate student has performed an in vitro pull-down assay to determine whether two proteins, Protein...
A graduate student has performed an in vitro pull-down assay to determine whether two proteins, Protein A and Protein B, interact with each other. Protein A is known to be 35 kDa and Protein B is 60 kDa. To determine whether there is an interaction the graduate student runs the sample down an SDS-PAGE gel, which is later stained with instablue dye. Unfortunately, the SDS, found in both the lysis buffer and SDS-PAGE gel, has expired (and is no longer...
A graduate student has performed an in vitro pull-down assay to determine whether two proteins, Protein...
A graduate student has performed an in vitro pull-down assay to determine whether two proteins, Protein A and Protein B, interact with each other. Protein A is known to be 35 kDa and Protein B is 60 kDa. To determine whether there is an interaction the graduate student runs the sample down an SDS-PAGE gel, which is later stained with instablue dye. Unfortunately, the SDS, found in both the lysis buffer and SDSPAGE gel, has expired (and is no longer...
Background: As we discussed in class, there are several complement regulators on the surface of our...
Background: As we discussed in class, there are several complement regulators on the surface of our cells and in our body fluids. These regulators are essential in preventing the activation of the complement cascade and eventual lysis of our self cells. In some cases, however, cells may become deficient in these regulatory proteins as a result of a random gene mutation in one of the pluripotent stem cells in the bone marrow. One such deficiency is called paroxysmal nocturnal hemoglobinuria....
Background: As we discussed in class, there are several complement regulators on the surface of our...
Background: As we discussed in class, there are several complement regulators on the surface of our cells and in our body fluids. These regulators are essential in preventing the activation of the complement cascade and eventual lysis of our self cells. In some cases, however, cells may become deficient in these regulatory proteins as a result of a random gene mutation in one of the pluripotent stem cells in the bone marrow. One such deficiency is called paroxysmal nocturnal hemoglobinuria....
biomaterials 2. You are performing an in vitro test in which you are testing the adsorption of three proteins (X, Y, and Z) to a material. After you have exposed the proteins to...
biomaterials
2. You are performing an in vitro test in which you are testing the adsorption of three proteins (X, Y, and Z) to a material. After you have exposed the proteins to the material surface, how do you determine which proteins adsorbed? What assay(s) would be suited to analyze the solution and the material surface
2. You are performing an in vitro test in which you are testing the adsorption of three proteins (X, Y, and Z) to a...
4. Bradford assay. In your prac you used the Bradford assay to determine the protein concentration...
4. Bradford assay. In your prac you used the Bradford assay to determine the protein concentration (1.5 marks) a) Explain at a molecular level how the color development of the assay works. Answer must be less than 100 words. (0.5 marks) b) Explain why you can use Bovine Serum Albumin as standard protein to determine the concentration of milk proteins. Answer must be less than 50 words. (0.5 marks) c) Why is it important to use diluted milk fraction samples...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme . Discuss how you were able to determine which protein...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme
. Discuss how you were able to determine which
protein was in which lane. You will have to do some
online research to find the molecular weight of each protein,
whether it is a dimer, etc. For at least one of the proteins, there
is some variability regarding the molecular weight, but the range
should still allow you to determine which lane each of the proteins
is in. For each lane, pay attention to...
Following an experiment in lab, you would like determine the number of arrangements possible for proteins...
Following an experiment in lab, you would like determine the number of arrangements possible for proteins adsorbing onto a surface. Consider 6 proteins adsorbing onto a 6 x 6 grid. The protein we're dealing with has long rigid 'arms'. As a result, once a protein adsorbs onto a site, no other protein can subsequently lie in the same row or column (diagonals are ok!) a) What is the total number of ways the 6 proteins can all adsorb onto the...
You have obtained 150 µL of a protein sample of which you want to perform a...
You have obtained 150 µL of a protein sample of which you want to perform a Bradford Assay. You perform a 10-fold dilution of your sample and use 10 µl of this dilution to quantify the amount of protein using a Bradford assay. You obtain an absorbance value of 0.63. Using the standard curve and equation you obtained in class, determine the concentration of protein (µg/µL) of your original sample. Show your work. equation: y=0.0139x-0.0153
You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine...
You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine albumin as your standard. You determine the Protein A concentration to be 2 mg/ml. When you use Protein A as your standard, you determine the Protein A concentation of your unknown to be 3 mg/ml. Explain the discrepancy (pick ONE answer): (2 points) a. Protein A has fewer basic amino acids than albumin. b. Protein A has more acidic amino acids than albumin c....
biomaterials
2. You are performing an in vitro test in which you are testing the adsorption of three proteins (X, Y, and Z) to a material. After you have exposed the proteins to the material surface, how do you determine which proteins adsorbed? What assay(s) would be suited to analyze the solution and the material surface
2. You are performing an in vitro test in which you are testing the adsorption of three proteins (X, Y, and Z) to a...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme
. Discuss how you were able to determine which
protein was in which lane. You will have to do some
online research to find the molecular weight of each protein,
whether it is a dimer, etc. For at least one of the proteins, there
is some variability regarding the molecular weight, but the range
should still allow you to determine which lane each of the proteins
is in. For each lane, pay attention to...
Following an experiment in lab, you would like determine the number of arrangements possible for proteins adsorbing onto a surface. Consider 6 proteins adsorbing onto a 6 x 6 grid. The protein we're dealing with has long rigid 'arms'. As a result, once a protein adsorbs onto a site, no other protein can subsequently lie in the same row or column (diagonals are ok!) a) What is the total number of ways the 6 proteins can all adsorb onto the...
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