Question

1. Deficiency of this molecule results in angioedema: ? a. IL-2 b. CI INH c . properdind . decay accelerating factor 2. Which is a biologic function of complement? a. promotes antigen presentation to CD4+ cells b. opsonin-mediated phagocytosis c. retention of immune complexes d. inhibition of histamine release 3. Which method quantifies antigen: a. Ouchterlony technique b.radial immunodiffusion c. Westem blot d. SDS-PAGE 4. IgG antibodies are predominant in causing? a negative selection of thymocytes b. secondary immune response c. isotype switching d. somatic recombination 5. The indirect Coombs' test is used to detect: a. desensitized RBCS b. RBCs sensitized with IgG c. IgM antibodies bound to blood group antigens d. IgG antibody that reacts at 37C 6. The ELISA technique that detects antigens in body fluid is: a. biotin-streptavidin b. sandwhich c. indirect d. nephelometry 7. Which of the following blood groups has anti-A circulating in the blood? a. A bB c. O d. AB e. b and c 8. A line of precipitation in immunodiffusion will not occur when there is: a. twilight zone b. zone of equivalence C. post-zone d. enzyme specificity for a substrate 9. Which condition is there are free antigen and free antibody in solution? a. prozone b .zone of equivalence c. postzone d. a and c 10. An agglutination reaction at 21C (room temperature) shows many very small clumps of RBCs in the test tube observed macroscopically. This is graded as: a. 1+ b. 2+ c. 3+ d. 4+ 11. Specificity of the beta chain of the TCR is determined by the: a kappa chains b. CD3 complex c. VDJ region d. Fab site 12. Antibody excess relative to Cantibody) will result in: a. a pro-zone phenomena b. lattice structure between antibodies and antigens C. opsonization d. the zone of equivalence 13. The correct percent RBC suspension in 0.9% saline used in serologic testing in immunohematology is: a. Unknown because it is determined by the manufacturer b. 10-20% c. 0.5-1.5% d. 2-5% 14. Identify the correct ABO group based on the following reactions: anti-A=0; anti-B-0 anti-D a. AB negative b. B negative c. O positive d. O negative
Answer as much as you can please

Answer 1

1. b.C1INH The lack of this esterase keeps the complement system constantly activated which leads to formation of bradkynin which dilates blood vessels and causes angioedema.

2.b.opsonin mediated phagocytosis . Antibody contains receptors for opsonin , and opsonisation is is done by complement mediated system.

3.b. Radial immunodiffusion is used to quantify antigen by measuring the thickness of precipitation zone it forms upon reacting with the antibody in wells made on a gel.

4. b. Secondary immune response. It acts against antigens from bacteria, virus etc. which have previously attacked the host.

5. RBCs sensitised with IgG in vitro

6. Sandwich elisa. In this technique the antigen in the fluid is captured in between two antibodies, one of which is lined to a conjugate.

7. e. Both B and O blood groups have anti -A circulating. O does not have any antigens on RBC and B has B antigen hence they have antibodies against A which is not present in their RBCs.

8. Postzone. Excess antigen prevents interaction with antibodies

9. d. Both postzone and prozone. Prozone is when antibody is in excess and postzone is formed when antigen is in excess

10. a.1+. Very small clumps are graded 1+ and large agglutinates are graded 4+. Agglutination occurs best between 4- 20 degrees

11.b. CDR3 complex is responsible for specific binding of TCR to antigen

12. Antibody excess will result in prozone phenomena.

13. d. 2-5% is ideal to detect antibodies resent in low amounts .

14. Since anti A, anti B are 0 RBCs do not have an antigen hence it Is O group. Since Anti D is present it is C. o positive