Question

4) Make a sketch of a gel after SDS-PAGE successful experiment performed with the 5 samples containing the following proteins and the mixed Markers that are shown below in the picture: Lysozyme 14 kDa Carbonic anhydrase 31 kDa Ovalbumin 45 kDa Bovine Serum Albumin 66 kDa Mysin 200 kDa ColorPlus Prestained Protein Marker, Broad Range APPARENT PROTEIN SOURCE MW (kDa) MBP-B-galactosidase 175 E COW MBP-truncated-3-galactosidase E.coli MBP-CBD Ecol CBD-Mxe Intein-2CBD [ Chi CBD-More Intein E.coli 30 CBD-E. coli par E.coli Lysozyme chicken egg white Aprotinin bovine lung MBP, maltose-binding protein, MBP-B-galactosidase fusion of MBP and B-galactosidase. MBP-truncated-3-galactosidase fusion of MBP and a truncated 3-galactosidase. MBP-CBD fusion of MBP and chitin binding domain CBD-M ini-2CBD fusion of the chitin binding domain, binein followed by two chitin binding domains CBD-Ecoparfusion of the chitin binding domain followed by and E. coli parvulin-like protein 10-20 SOS PAGE

Answer 1

Marker lane 2 lysozyme angolrise ovalbumin Boimalla Korthin IIIIIIIII

protein which are larger in size travel less and those with smaller size travel fast.

SDS- PAGE protein are separated on the basis of the size and not charge because SDS provide the uniform negative charge to all the proteins.