Homework Answers
Tributyrin agar is a differential medium that trsts the ability of an organism to produce an exoenzyme called lipase that hydrolyzes tributyrin oil. The inability of this medium to distinguish between different enzymes is not a weakness of the medium because the function of tributyrin agar is to detect lipolytoc microorganisms such as Clostridia, Pseudomonas etc through formation of clear zones surrounding lipolytic colonies. It is classified as a differential medium because it can differentiate between lipases as well as it posess the ability differentiate between the organisms with or without lipases.
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Many organisms possessing many different lipases produce positive results on tributyrin agur. Is the inability of...
1. What are the different sources of energy available to living organisms? 2. How do the...
1. What are the different sources of energy available to living organisms? 2. How do the acquisition and the use of energy by living organisms work according to the laws of thermodynamics? 3. Explain the energy use in the following reactions: endergonic/exergonic. 4. What is metabolism? How are chemical reactions related to metabolism? Why is energy needed to run a metabolism? What are coupled reactions? 5. Draw a picture of ATP. Why is this molecule so important for cells? How...
Question 1: Homeostasis, Common Ancestry, and Divergence a) One problem that all respiring organisms share is...
Question 1: Homeostasis, Common Ancestry, and
Divergence
a) One problem that all respiring organisms share is the
need for biological structures that allow efficient uptake of
oxygen and removal of carbon dioxide. Two types of structures that
have evolved for carrying out gas exchange are gills and lungs. The
table below lists organisms according to the type of structure they
use for gas exchange.
Describe the general process of gas exchange that occurs
between a gill or lung and the...
You are given a mixed culture of two organisms in a small tube of TSB.
You are given a mixed culture of two organisms in a small tube of TSB. These two organisms include a Gram-negative strain (your bacterial strain to identify) and a Gram-positive strain (a contaminant). Your first task is to isolate these organisms which will help you identify which Gram-negative strain you are working with. 1) You inoculate two streak plates on TSA media and MacConkey Agar using aseptic technique. For each medium, state whether the medium is selective, differential, or neither and...
can somrone help me write an introduction for each agar. thank you ? Introduction: Selective and...
can somrone help me write an introduction for each agar. thank you
?
Introduction: Selective and differential media are used frequently in Microbiology Pathology Labs to identify a known group of bacteria from a complex mix of other bacteria. The medium is designed to select against as many of the background organisms as possible by using a medium in which they will not grow but the organisms under investigation can grow hence the "Selective" part of the name. Any assayable...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in the "unculanes? If there are more than one bad explain the nature or origin of the other bands? If there are more than one band can you 2) Why did you have to electrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of pBR322 with the enzymes you used? 4) What are the sizes of the...
polymerase chain reaction, many copies of regions of the genome for a number of different downstream...
polymerase chain reaction, many copies of regions of the genome for a number of different downstream applications. In this Project you are PCR-amplifying your mitochondrial HVR1 region so that you can obtain the DNA sequence for that region. The ultimate goal is to determine your haplotype or haplogroup based on that region, which will provide you with insight into your deep ancestry. consider the class discussions you had on PcR this week. Use this information to answer the following questions:...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that raise the pH to 7.8. The starting pH of EMB plates should be 6.9 – 7.3. No one is aware of the change. The EMB plates are inoculated for identification of unknown bacteria. After the appropriate incubation time, the results are interpreted. A. Discuss the consequences of the change in starting pH to the interpretation of the results. (The answer is not that no...
Can you think of a biological reason why so many different cultures around the world converged...
Can you think of a biological reason why so many different cultures around the world converged on the idea that mating between close relatives should be discouraged (this started long before anyone knew about genetics)? Explain your answer.
Metabolic Pathway Engineering Problem Set 5 Engineering a Fermentation System: Fermentation of plant matter to produce...
Metabolic Pathway Engineering Problem Set 5 Engineering a Fermentation System: Fermentation of plant matter to produce ethanol for fuel is one potential method for reducing the use of fossil fuels and thus the CO2 emissions that lead to global warming. Many microorganisms can break down cellulose then ferment the glucose to ethanol. However, many potential cellulose sources, including agricultural residues and switchgrass, also contain substantial amounts of arabinose, which is not as easily fermented. Escherichia coli is capable of fermenting...
values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret...
values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret Test Color with Biuret Reagent Test Tube Contents Questions: 1) Which tubes contain protein? Describe a positive biuret test for protein based on your experimental results. the Rpomin Cole tor 丹 ts in proteins 2) Based on your qualitative (visual) observations of the Biuret test results, do all three tubes contain the same amount of protein? How do you know? 3) Explain method by...
1. What are the different sources of energy available to living organisms? 2. How do the acquisition and the use of energy by living organisms work according to the laws of thermodynamics? 3. Explain the energy use in the following reactions: endergonic/exergonic. 4. What is metabolism? How are chemical reactions related to metabolism? Why is energy needed to run a metabolism? What are coupled reactions? 5. Draw a picture of ATP. Why is this molecule so important for cells? How...
Question 1: Homeostasis, Common Ancestry, and
Divergence
a) One problem that all respiring organisms share is the
need for biological structures that allow efficient uptake of
oxygen and removal of carbon dioxide. Two types of structures that
have evolved for carrying out gas exchange are gills and lungs. The
table below lists organisms according to the type of structure they
use for gas exchange.
Describe the general process of gas exchange that occurs
between a gill or lung and the...
can somrone help me write an introduction for each agar. thank you
?
Introduction: Selective and differential media are used frequently in Microbiology Pathology Labs to identify a known group of bacteria from a complex mix of other bacteria. The medium is designed to select against as many of the background organisms as possible by using a medium in which they will not grow but the organisms under investigation can grow hence the "Selective" part of the name. Any assayable...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in the "unculanes? If there are more than one bad explain the nature or origin of the other bands? If there are more than one band can you 2) Why did you have to electrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of pBR322 with the enzymes you used? 4) What are the sizes of the...
polymerase chain reaction, many copies of regions of the genome for a number of different downstream applications. In this Project you are PCR-amplifying your mitochondrial HVR1 region so that you can obtain the DNA sequence for that region. The ultimate goal is to determine your haplotype or haplogroup based on that region, which will provide you with insight into your deep ancestry. consider the class discussions you had on PcR this week. Use this information to answer the following questions:...
values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret Test Color with Biuret Reagent Test Tube Contents Questions: 1) Which tubes contain protein? Describe a positive biuret test for protein based on your experimental results. the Rpomin Cole tor 丹 ts in proteins 2) Based on your qualitative (visual) observations of the Biuret test results, do all three tubes contain the same amount of protein? How do you know? 3) Explain method by...
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