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target dna is integrated into a plasmid inserted into bacteria and allows for selection of transformed...

target dna is integrated into a plasmid inserted into bacteria and allows for selection of transformed bacteria colonies

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Transformation is a key step in DNA cloning. DNA of interest is cut down by restriction enzymes and then ligated into a plasmid and then this plasmid is tranferred into the bacteria. Bacteria are selected on antibiotic plates. Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony.

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