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8. Human blood contains two buffer systems, one based on phosphate species and one on carbonate species. At the temperature o
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a. Human blood at its normal temperature has the pKa values for the phosphate species are 1.89,6.64 and 11.22.

Then as such, the pKa value for carbonic acid species is 6.10 and 9.80.when the pH 7.4 falls within + or - 1 pH unit of phosphate is pKa2 of 6.64, it is expected that H2PO4- and HPO42-' is present significantly and H3PO4 or PO43- is present in little.

b. The ratio between two phosphate species is calculated as

7.4 = 6.64 + log [HPO42-]/ [H2PO4-]

Thus the ratio is [HPO42-]/ [H2PO4-] = 5.8.

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