Question

What is the importance of GE protein? Yes, it is called GE. It is a sensor for macromolecular crowding and ionic strength in cells. Use background information to illustrating the broader impact. Here are some sources since you are obviously struggling. DOI 10.1021/acschembio.7b00348 DOI 10.1021/acs.jpcb.7b01306

Cerulean(GSG) AEAAAR) A{GSG) AEAAAR)A(GSG), Citrine M modified from Boersma et al. Nature Methods (2015) 12:227

Answer 1

The above mentioned GE protein is basically a Forster resonance energy transfer (FRET) sensor for quantifying the molocular dynamics. Here, excited-state dynamics and translational diffusion of the FRET sensor were evaluated. The probe with a shorter linker, G18, was shown with high transfer efficiency. This sensor is used for the monitoring the spatiotemporal changes in the ionic stregth inside a cell, in different study. Altogether, this sensor helps in sensing the polarization imaging and ionic strength at the macromolecular crowding sites inside the cells.