Homework Answers
Bactrial
conjugation will be happens F+ bacteria(Donor) contains pilus and
chromosomal DNA , plasmid DNA for F- strains(receptor) contain only
chromosomal DNA
Once conjugation will be pilus and attached to another bacteria plasmid DNA will be traveling to receptor bacteria and final strains will be F+,F+
Ampcilin and streptomycin antibiotics resistance will be streptomycin will be high
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1. Using the example genetic strains in this exercise draw a diagram illustrating two bacteria conjugating....
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of E.coli (streptomycin-sensitive Hfr strain and a streptomycin-resistant F-straint, aliquots were extracted from the mating mixture at various time points and plated on minimal media supplemented with streptomycin and a variety of amino acids. The number of conjugates at each time point on each type of media are shown in the table below. What can you conclude from these data!! Time (minutes) Medium MM (-)...
thats why i was so confused on this one, because our teacher did not give us...
thats why i was so confused on this one, because our teacher did
not give us any surrounding context regarding experimental
biotechnology
1) You are given 2 strains of E. coli, X & Y. Strain X carries a kanamycin (kan) resistance gene on a plasmid and strain Y carries a tetracyline (tetR) resistance gene on a plasmid. The 2 strains are mixed together, plated on media containing kanamycin and tetracyline and incubated. The next day you notice that there is...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
I really need help with these questions!! 2. Bernard Davis tested the "cross-feeding" interpretation of some...
I really need help with these questions!! 2. Bernard Davis tested the "cross-feeding" interpretation of some data that showed the phenotype of one microbe as capable of being changed by another microbe. His contribution can be summarized as: A) using one strain of microbes as food for another, and then evaluating the impact upon microbial phenotype. B) combining microbes with complementing auxotrophs in a single tube and then analyzing the cells of gene exchange. C) placing a barrier between bacterial...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of E.coli (streptomycin-sensitive Hfr strain and a streptomycin-resistant F-straint, aliquots were extracted from the mating mixture at various time points and plated on minimal media supplemented with streptomycin and a variety of amino acids. The number of conjugates at each time point on each type of media are shown in the table below. What can you conclude from these data!! Time (minutes) Medium MM (-)...
thats why i was so confused on this one, because our teacher did
not give us any surrounding context regarding experimental
biotechnology
1) You are given 2 strains of E. coli, X & Y. Strain X carries a kanamycin (kan) resistance gene on a plasmid and strain Y carries a tetracyline (tetR) resistance gene on a plasmid. The 2 strains are mixed together, plated on media containing kanamycin and tetracyline and incubated. The next day you notice that there is...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
.2 . . .3 Agar + Kan Agar + Pen Agar + Kan + Tet Agar + Kan + Amp Agar +Tet + Amp Agar + Pen +Kan Agar +Tet + Pen 2. Strains I and II undergo conjugation and colonies from the "mating plate are put on the following agar plates with antibiotics. Look at the results in the following table: 1 Strain III + E S Plain agar38832 Agar + Ampe SENS Agar +Tet Agar+Kan Agar-Pen 03 Agar...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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