Question

4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X 0 50 10 20 30 40 time (minutes) -strR +hmm+ poo+ omg+ b) If you attempted to grow your exconjugants at 10 minutes on minimal media, what other supplement would you have to add to that minimal media in order to see growth? c) Based on these data, draw a chromosomal map of your Hfr donor cell. Make sure you include in your drawing where the origin was located and indicate somehow the direction of gene transfer.

Answer 1

A) yes. streptomycin resistance gene is 10 minutes distant apart from the origin of transfer and all the genes are transferred in 20 minutes.so that it can grow the recipient exoconjugate in complete media with streptomycin

B) in 10 minutes poo, streptomycin resistant gene and hmm genes are transferred. we should use only genes that are not transferred in minimal media. Since omg gene is not transferred and we should grow it in minimal media with omg supplement so that we can see growth

C)

Ongen Cong) 110 minutes rs R ر / alspuim op NX shumt To min. : Poot