Homework Answers
100 micromolar
use formula c1vi=c2v2
here c1 is 100micromolar
v1 is 20mM
V2 is 20ml
and we find C2
make equal unit
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In step 2, we will be adding 100 uL of 20 mM IPTG onto...
**Answer D: Please show steps and explanations for each step** 4. A student makes the following preparation. She takes...
**Answer D: Please show steps and explanations for each
step**
4. A student makes the following preparation. She takes 400 grams of chemical A and add water to a final volume of 1 Liter. The molecular weight of chemical A is 200 grams/mole. This solution is referred to as solution 1. She then takes 2 ml of solution 1 and adds it to 8 mls of water and mixes it; this is solution 2. She then take 5 ml of...
Please solve A B and C. Show each step to the solutions. I attached the answers...
Please solve A B and C. Show each step to the solutions. I
attached the answers for B but am not sure how to get to these
solutions.
please solve A B and C. Show each step to the solutions 6.82. A salt A is soluble in a solvent S. A conductivity meter used to measure the solute concentration in A-S solutions is calibrated by dissolving a known quantity of A in S, adding more S to bring the solution...
PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone,...
PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone, 0.5% yeast extract, 10 mM NaCL (F.W. 58), 2.5 mM KCl (F.W. 74.5), 10 mM MgSO4 (F.W. 246.5), 10 mM MgCl2 (F.W. 203.3), 20 mM glucose (F.W. 180.16). Determine the amounts of each to make 500 mL of SOC. 2) Determine the number of moles in 0.05 μg of 300 bp insert DNA. There are 660 g/mol-bp. What is the molar ration of insert...
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20...
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20 mL of 100 mM NaCl to your assay, and the final volume of the assay is 100 ml. What is the working concentration of NaCl? The number of significant digits you include in your answer doesn't matter, but it must be in units of mM. QUESTION 2 Imagine you are preparing an enzyme assay that requires a working concentration of 10 mM NaCl. If...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
This is what we did in the experiment .First of all,1 µl of plasmid DNA was...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
Just answer part 2 and 3 please (0.3 point) Part 1 ii See Periodic TableSee Hint A solution is created by dissolving 12.5 grams of ammonium chloride in enough water to make 355 mL of solution. How ma...
Just answer part 2 and 3 please
(0.3 point) Part 1 ii See Periodic TableSee Hint A solution is created by dissolving 12.5 grams of ammonium chloride in enough water to make 355 mL of solution. How many moles of ammonium chloride are present in the resulting solution? moles of NH4CI 0.233 When chemists work with solid materials, we simply weigh out amounts of solid reagents and calculate mole amounts when thinking about stoichiometry. However, when we dissolve a solid...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
**Answer D: Please show steps and explanations for each
step**
4. A student makes the following preparation. She takes 400 grams of chemical A and add water to a final volume of 1 Liter. The molecular weight of chemical A is 200 grams/mole. This solution is referred to as solution 1. She then takes 2 ml of solution 1 and adds it to 8 mls of water and mixes it; this is solution 2. She then take 5 ml of...
Please solve A B and C. Show each step to the solutions. I
attached the answers for B but am not sure how to get to these
solutions.
please solve A B and C. Show each step to the solutions 6.82. A salt A is soluble in a solvent S. A conductivity meter used to measure the solute concentration in A-S solutions is calibrated by dissolving a known quantity of A in S, adding more S to bring the solution...
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20 mL of 100 mM NaCl to your assay, and the final volume of the assay is 100 ml. What is the working concentration of NaCl? The number of significant digits you include in your answer doesn't matter, but it must be in units of mM. QUESTION 2 Imagine you are preparing an enzyme assay that requires a working concentration of 10 mM NaCl. If...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
Just answer part 2 and 3 please
(0.3 point) Part 1 ii See Periodic TableSee Hint A solution is created by dissolving 12.5 grams of ammonium chloride in enough water to make 355 mL of solution. How many moles of ammonium chloride are present in the resulting solution? moles of NH4CI 0.233 When chemists work with solid materials, we simply weigh out amounts of solid reagents and calculate mole amounts when thinking about stoichiometry. However, when we dissolve a solid...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
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