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Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
Sketch the predicted migration pattern on an agarose gel of the following DNA samples: (1) Undigested...
Sketch the predicted migration pattern on an agarose gel of the following DNA samples: (1) Undigested pET15b plasmid; (2) pET15b digested with the EagI restriction enzyme; (3) pET15b digested with NdeI and AhdI (see pET15b vector map for location of these enzyme cutting sites). Include in your sketch a DNA base-pair ladder from 0 to 10,000 bp with 1000 bp increments. Label each band in the DNA ladder with the known size (in base-pairs), and estimate the size of the...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
While your gel is running, discuss your expected results for each lane on the gel •...
While your gel is running, discuss your expected results for each lane on the gel • Remove the gel from the apparatus and image on a UV transilluminator. (Remember to only illuminate the UV light when the shield is in place). 3) Using the DNA ladder, estimate the experimental size of all bands observed in the sample lanes. (3pt) Gel Lane Plasmid Number of bands observed Estimated size (bp) of each band 2 (tube 1) A 3 (tube 2) B...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
hi I need help with these questions please q1 How long is the DNA sequence encoding...
hi I need help with these questions please
q1 How long is the DNA sequence encoding the protein that
confers ampicillin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q2 How long is the DNA sequence encoding the protein that
confers hygromycin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q3 What is the size of the OTC-Δ DNA sequence that has been
inserted into the...
colony which one express gene Only correct explanation not just answears pls. how to determine correct...
colony which one express gene
Only correct explanation not just answears pls.
how to determine correct orientation by electrophoresis
CORI -Hindi mal kpn ! Aggi -Hind III Gene CDNA EcoRI 5. G' AAT TC 3 CTTAAG. 5 Бра 5GGT ACC 3 3 CCATGG 5 pMAE2 Avr 11 M5 CCTAGGS 3 GGATCC.5 Arell ACCGGT 3 3.. TGGCCA...5 Amp Hind 1 5: AAGCTT 3 S 3. TTCGAA53 Xma C CGGG.3 GGGCGC 5 You try the following strategies in order to see if...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb in length. Lane "T" is a standard DNA ladder, not a digestion. (2 points total) I Gel lanes II III IV V Which of the following is TRUE? 20 K a) The enzyme used in lane III cuts 4 times b) The enzyme used in lane II cuts 1 time c) The enzyme used in lane IV cuts 3 times d) It is likely...
How did you EXPECT your uncut lane to look in the gel image? What ACTUALLY happened?...
How did you EXPECT your uncut lane to look in the gel image?
What ACTUALLY happened? What is a plausible explanation if there
was any discrepancy?
How did the observed BclI and EcoRI digest results compare to
the expected results? If they differed, list a potential reason
why?
BclI was incubated at 50°C, while EcoRI was incubated at 37°C.
Why was this necessary? What might you predict to occur if you
accidentally switched the temperatures?
3) Using the DNA ladder,...
Hi can someone help me understand part C and why the drawn in red lines are...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
While your gel is running, discuss your expected results for each lane on the gel • Remove the gel from the apparatus and image on a UV transilluminator. (Remember to only illuminate the UV light when the shield is in place). 3) Using the DNA ladder, estimate the experimental size of all bands observed in the sample lanes. (3pt) Gel Lane Plasmid Number of bands observed Estimated size (bp) of each band 2 (tube 1) A 3 (tube 2) B...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
hi I need help with these questions please
q1 How long is the DNA sequence encoding the protein that
confers ampicillin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q2 How long is the DNA sequence encoding the protein that
confers hygromycin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q3 What is the size of the OTC-Δ DNA sequence that has been
inserted into the...
colony which one express gene
Only correct explanation not just answears pls.
how to determine correct orientation by electrophoresis
CORI -Hindi mal kpn ! Aggi -Hind III Gene CDNA EcoRI 5. G' AAT TC 3 CTTAAG. 5 Бра 5GGT ACC 3 3 CCATGG 5 pMAE2 Avr 11 M5 CCTAGGS 3 GGATCC.5 Arell ACCGGT 3 3.. TGGCCA...5 Amp Hind 1 5: AAGCTT 3 S 3. TTCGAA53 Xma C CGGG.3 GGGCGC 5 You try the following strategies in order to see if...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb in length. Lane "T" is a standard DNA ladder, not a digestion. (2 points total) I Gel lanes II III IV V Which of the following is TRUE? 20 K a) The enzyme used in lane III cuts 4 times b) The enzyme used in lane II cuts 1 time c) The enzyme used in lane IV cuts 3 times d) It is likely...
How did you EXPECT your uncut lane to look in the gel image?
What ACTUALLY happened? What is a plausible explanation if there
was any discrepancy?
How did the observed BclI and EcoRI digest results compare to
the expected results? If they differed, list a potential reason
why?
BclI was incubated at 50°C, while EcoRI was incubated at 37°C.
Why was this necessary? What might you predict to occur if you
accidentally switched the temperatures?
3) Using the DNA ladder,...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
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