Explain why measuring the intracellular amount of Glutamate would be a poor way to determine whether...
Explain why measuring the intracellular amount of Glutamate would be a poor way to determine whether a neuron normally releases Glutamate during neurotransmission What are two other things you might measure to determine whether the neuron normally releases Glutamate?
Homework Answers
The intracellular glutamate concentration can be due to previous release of neurones or through other secondary pathways like amino acid metabolism.
1. fluorescent indicator protein for glutamate (FLIPE) is a genetically encoded nanosensor. It consists of a glutamate/aspertate binding protein from E.coli which is fused to two variants of green fluorescent protein. When glutamate is present there is a decrease in FRET efficiency as the two fluorescence Protein can no longer bind.
2. Measuring the amount of secondary effectors in the pathway of glutamate signalling is another option. Glutamate in astrocytes activate NMDA receptors which results in calcium spike. Measuring amount of calcium level in nerve cells can aslo be an indirect way.
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