Question

1. Our study uses CRIPSR targeting vectors to the ALK and ELM4 genes.
   1. What is the ALK gene and normal function (include signaling pathway)? - Anaplastic lymphoma kinase is an enzyme that is encoded by the ALK gene in humans. It plays a significant role in cellular communication, normal development and function of the nervous system, and plays crucial roles in adult behavior.
2. 2. What type of cancer is associated with mutation of the ALK and EML4 genes? - It is associated with lung cancer
3. 3. What type of mutation is this? - Inversion of chromosome 2 that fuses together (via translocation) the EML4 (through the 5’ end) and ALK (through the 3’ end) gene that indicates to a division of non-small cell lung cancers (NSCLC).
   4. Describe at a molecular level how this mutation from part c is associated with cancer?
   5. Using the knowledge above from part d, how could you screen for cells if our CRISPR constructs generate this type of mutation if? Include expected data from this analysis?

I only need d & e

Answer 1

improvement in outcome of lung cancer is likely to be achieved by identification of molecular levents that underlie its pathogenesis. Small inversion of chromosome 2p results in formation of fusion gene comprising portion of echinoderm microtubule assossiated protein like eml4 gene and alk gene nsclc cells.

3-7 % of nscls are alk positive , 21 known eml 4-alk breakpoint variants exists, rare fusion exists with tgf,klc1,k1kf5b