Question

1. Prepare two tubes. Label one '+' and the second . Add 5 pl of your total RNA to each. Using the supplied tube of 1M TRIS PH 7.5 make each tube up to 6 pl. The final concentration of TRIS should 25 mm. (Hint: determine the concentration of TRIS you need to make to add 1 pl to your RNA samples; i.e., think about what you calculate and what micropipettors you have available to you. What volumes do they accurately pipette?).

Answer 1

Page 1 Aceading to the given införation, step:-1, T To add loul to this buffer to the RNA Sou 15.ould so that the total volume become bioplę the firal becomes a5.0mm. let the core of desired Tris sole be a Now, Using Cil, = C, V2 cixliop! = 25 mM x biopel o c=(25mMx topl/hopel = 150mm Herce, in the desired Tois solue = 150mm = 0.150M step: a We have steck .om Desisad = 6:150m [fzom i stop) 2- top / micropipettes (generally minilable in mol bio labs).

sequured ulume LOS let the required udurne of the draged touis sodu besonel Appuis Using Cr, - Cave op, Ve= (0.150Mx zope!) 10M = 3.Opel So, sequeried wole of stock tocis elu - 3. omel. And, segured when of sterile, deionized water stock 17. O = popel - 3 Oml = 17.opel # papaantion derived on some Turis solui- dud tube, add zione! a steule Tante Tous" schia! To this, add 17.00 there ne Learn be decenijed woker. This is og solution ºf 0.150 M. please give a positive vote