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)3.Strains of E. coliBL21 often have extra plasmids (i.e. codon plus, pLysS) in addition to the...

)3.Strains of E. coliBL21 often have extra plasmids (i.e. codon plus, pLysS) in addition to the one that you are transforming into this strain. How could this affect plasmid miniprep isolation of your desired plasmid from this strain in comparison to isolating your plasmid from the DH5alpha subclone?
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There is difference between Ecoli DH5alpha and Ecoli BL21DE3

Ecoli Dh5alpha is used for transformation studies followed by plasmid miniprep to measure the concentration of DNA and it produces huge concentration of DNA in terms of ng/microlitre in Nanodrop

Whereas Ecoli BL21DE3 is used for expression studies i.e it expresses protein and the protein produced from this is less,so to produce more we check the type of protein whether it is intracellular or extracellular and carry ultrasonication and carry AKTA to produce purest form of protein which can be scaled up.

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