Question

can you please answer all three questions. Thank you so much!!

QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks]

Answer 1

Answer & purpose of TAE A buffer resistant pr change.. A biological buffer is a combination of a week acid and - conjugated base or vice versa Help to maintain costantin of the solution * TAE is needed to maintain the pH of DNA solution of we used water instead of TAE. Then due to electric currents water can dissociare a release ritims. DNA-ve charge tHtims DNA Neutral No more Movement So that's why use buffer instead of water 0 - * Agarose gel concentration - migratim rate of DNA molecules affected by the concentration of gel. The migration rate of a DNA Molecue decrease when conse of agarose in the gel increase . Agarose gel concentration increase » pore size decreases DNA molecule movement is slow because of smay size of pores Commonly t% get used for separation of DNA → » 4mya @ cmtемент (n(x o') - Pom< size 11 - 14тех учң can rescine (10-30kb) а т . нач (x21) - Рок «Д - фаце и I can resolve Cloobp - 2000 bp) factors. Time Time also affects the resolution of DNA . Because when if Je will give more time (greater than a 4.30 hr). Them DNA fragments Move to release in buffer ... a if tracking dye yoves down almost 70-80% then should be switen off electrophoretic unit if gel concentration High → DNA Molecure takes more time to separate for 3. Voltage - If voltage is high then DNA fragments move fast but high voltage also decrease the resolution. - le voltage is high from limited then sometimety ultimately causes to gelto melt voltage range → 75-1sou . it you run your gel at lower voltages (75-85), your bands will. clearer than Higuer voltages ...

Answer 2. DNA fragnents move towards the positive end of the gel box. Because DNA fragments have negative charge due to presence of p3 pmc aia (Pophae тір)