Question

V.O. What are the basis of separating different compounds by gel filtration chromatography and von is by gel filtration chromatography and ion exchange anromatography? Please briefly describe similarities and differences in these two tego The basis of seperating different Compands by gel filtration Chromate and on exchange Chromatography a

Answer 1

The principle behind gel filtration chromatography is the separation of molecules on the basis of their size. Whenever mixture of different substances is made to pass through the stationary column, then the substance having smaller molecules interact with the stationary phase and will be retarded and the substance having larger molecules will not interact with the stationary phase and will come out first from the column.

Ion exchange chromatography works upon the principle of affinity for Ion exchanger. A solution having a mixture of cations and anions is made to pass through the stationary column, the cations bind to anions and anions bind to cation. Cations are present in the solution mixture are taken out by adding a solution from outside which is rich in cations. These cations bind to the anions of stationary phase and help in the coming out of the cations of solution mixture. Similarly anions are also removed from the chromatography column.

The similarities between these two processes are -

Both of them use a packed chromatography column for the separation of solution mixture.

Both of them can separate two types of molecules in one go.

Both of them require specific solution for the recovery of tenses present in the mixture after separation.

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