alculate the value Vo if an enzyme has Km=5.4 x 104. V max=484M/min and [S] =...

Question

Question

science biology

Answer 1

Answer #1

1) according to Michelis Menten equation

Vo=( Vmax X S)/ (Km+S)

Vo=(48uM/secx1000uM)/(5.4x10^-4M+0.001M)

Vo= 48x10³uM²/sec/15.4x10^-4M

Vo=48x10³uM²/sec/1540uM

Vo= 48000/1540 uM/sec

Vo=31.16 uM/sec

2) Vo= (Vmax X S )/ (Km+ S)

6um/sec= 35um/sec X 3x10^-5M/(Km + 3X10^-5 M)

Km + 3x10^-5 M =17.5x 10^-5M

Km=14.5x10^-5M

Km=145uM

Hope it's clear..thanks

Answer 2

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