If you are provided with a CHO cell line expressing an adrenergic receptor, how could you...
If you are provided with a CHO cell line expressing an adrenergic receptor, how could you experimentally demonstrate that this receptor coupled to increases in cAMP via a G protein?
Homework Answers
The induced form of G coupled plasma was found in certain legands , it linked in certain inactive form . So, they communicate with signalling with other receptor with the help of G proteins .
The over expressed form of adrenergic receptor are seen , with this there is a continuous increase in form of GPCRs form was observed.
For expressing the adrenergic receptor, cho cell line characteristics of cell line is observed.
The process can be fluorescent ligands specially recognise adrenergic receptor by expressing with G Coupled receptor.(gpcr) Chinese hamster ovary( cho).these usually aceppted expressing simple no of Recombinant DNA.
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