You have to add Ethidium Bromide to an agarose gel at 1µg/mL. The Ethidium Bromide stock...

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You have to add Ethidium Bromide to an agarose gel at 1µg/mL. The Ethidium Bromide stock...

You have to add Ethidium Bromide to an agarose gel at 1µg/mL. The Ethidium Bromide stock solution has a concentration of 10 g/L. How much of the Ethidium Bromide stock do you have to add to 150 mL of the gel? Give volume in µL.

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Answer 1

Answer #1

The ethidium bromide stock solution concentration is 10g/L based on working concentration 10mg/ml

Based on gel electrophoresis we have to had working concentration will be 150ml * 0.5 ul=75ul we have to add and make up to final volume 150ml.

150*0.5 ul=75 ul

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Answer 2

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You have to add Ethidium Bromide to an agarose gel at 1µg/mL. The Ethidium Bromide stock...

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