Homework Answers
Parameters to be considered from the graph :
- Coeffcient of determination r^2 = .9843 which indicates that that variation of 98.43 percent of variation in absorbance can be explained by variation in concentration of the sample.
- x = concentration of glucose
- Absorbance Value y =
- Slope m = 1.0357
- Concentration of Glucose b = .71
The regression line between the absorbance values (y) and the concentrations (x) was found by minimizing the sum of the squares of the residuals so as to find the slope (m) and the y-intercept (b). The values obtained were m = 1.0357 and b=-0.071
Absorbance =1.0357 x-0.71. (substitute x with glucose concentration or take a mean of all and use)
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You have just created a standard curve of glucose concentration as a function of ANS absorbance...
Using the calibration curve, calculate the molar concentration for a solution with a measured absorbance of...
Using the calibration curve, calculate the molar concentration for
a solution with a measured absorbance of 0.143.
Concentration = M
0.7 0.6 y = 0.337x 0.5 0.4 Absorbance 0.3 0.2 0.1 o o 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 Concentration, M
In a glucose standard curve, a solution with zero glucose measures zero absorbance on a spectrophotometer,...
In a glucose standard curve, a solution with zero glucose measures zero absorbance on a spectrophotometer, and a solution with 50 mmol/liter glucose measures 0.8 absorbance units. The standard curve is linear up to 200 mmol/liter glucose. What absorbance should a solution with 100 mmol/liter glucose have? 0.4 1.6 1.2 0.8
Here are the graphs. instrument should have an absorbance reading of zero when there is no...
Here are the graphs.
instrument should have an absorbance reading of zero when there is no CuS04 present. Print copies of the graph for you and your partner to submit with your lab reports. Calculate the concentration of the unknown using the equation for the line. emros Concentration (M) Absorbance 0.5 1.005 0.3 0.704 0.617 0.25 0.1 0.291 0,05 0.131 0.513 Concentration VS Absorbance 1.2 y 2.1773x R2 0.9566 1 e4e4eeresases4s4 0.8 0.6 0.4 0.2 0.4 0.5 0.6 0.1 0.2...
The figure below depicts the absorbance of the antibiotic Ampicillin at 610 nm as a function of its concentration (...
The figure below depicts the absorbance of the antibiotic Ampicillin at 610 nm as a function of its concentration (in ug/mL). The equation for the line of best fit was: y 0.01054+0.1606 Determine the concentration of an unknown sample of Ampicillin, if its absorbance at 610 nm was 0.450 0.5 0.45 0.4 0.35 0.3 0.25 0.2 15 20 Concentration ug /ml 25 10 Absorbance
3. In Part A, you measure the absorbance of four FesCN" solutions and prepare a plot...
3. In Part A, you measure the absorbance of four FesCN" solutions and prepare a plot (Absorbance vs. concentration) similar to the one below. Beer's Law Plot for FeSCN2 0.6 0.5 0.4 0.3 d 0.2 0.1 0 y = 2417.5× + 0.0483 R2 0.99897 0.00025 0.00015 0.0002 0.00005 0.0001 0 Concentration (M) You use this plot as a standard curve in Part B to determine the concentration of an unknown. If an unknown FeSCN2 solution gives an absorbance reading of...
Calibration curve of dilutions 0.6 0.5 y = 8E-05x2 + 0.0189x R2 = 0.99615 0.4 Absorbance...
Calibration curve of dilutions 0.6 0.5 y = 8E-05x2 + 0.0189x R2 = 0.99615 0.4 Absorbance 0.3 0.2 0.1 0 0 5 10 15 20 25 30 Concentration Using this calibration curve, what is the concentration of the sample if the sample has its Absorbance 86.22 HTML Editor
using my standard curve I need to determine the concentration of glucose in the unknown the...
using my standard curve I need to determine the concentration of glucose in the unknown the equation is y=-0.1741x+0.7254 the absorbance for the unknown is 0.201
1. A researcher was evaluating the concenltaIO U a submerged fermentation. He prepared a stock so...
1. A researcher was evaluating the concenltaIO U a submerged fermentation. He prepared a stock solution of 0.1mg bovine Serum Albumin. Plot a graph of a standard curve using the following data sets and determine the concentration of the following proteins X, Y, and Z Concentration mg/ml 0.2 0.4 0.6 0.8 1.0 Absorbance nm 0.245 0.389 0.612 0.745 1.025 0.824 0.452 0.689 Convert the concentration of X, Y, and Z to g/l and %w/v.
1. A researcher was evaluating the...
using my standard curve I need to determine the concentration of glucose in the unknown Volume...
using my standard curve I need to determine the concentration of glucose in the unknown Volume of Glucose added (ml) Glucose Concentration (mol/l) % Transmittance Absorbance 0.00 2.7001 x 10-3 17.6 0.754 1.00 2.7001 x 10-5 31.2 0.506 2.00 5.4002 x 10-5 41.5 0.382 3.00 8.1003 x 10-5 60.9 0.215 Unknown 63.0 0.201 Using the standard determine the concentration of glucose in the unknown. The equation is y=-0.1741x+0.7254. this is all the information thats needed
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
Using the calibration curve, calculate the molar concentration for
a solution with a measured absorbance of 0.143.
Concentration = M
0.7 0.6 y = 0.337x 0.5 0.4 Absorbance 0.3 0.2 0.1 o o 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 Concentration, M
Here are the graphs.
instrument should have an absorbance reading of zero when there is no CuS04 present. Print copies of the graph for you and your partner to submit with your lab reports. Calculate the concentration of the unknown using the equation for the line. emros Concentration (M) Absorbance 0.5 1.005 0.3 0.704 0.617 0.25 0.1 0.291 0,05 0.131 0.513 Concentration VS Absorbance 1.2 y 2.1773x R2 0.9566 1 e4e4eeresases4s4 0.8 0.6 0.4 0.2 0.4 0.5 0.6 0.1 0.2...
The figure below depicts the absorbance of the antibiotic Ampicillin at 610 nm as a function of its concentration (in ug/mL). The equation for the line of best fit was: y 0.01054+0.1606 Determine the concentration of an unknown sample of Ampicillin, if its absorbance at 610 nm was 0.450 0.5 0.45 0.4 0.35 0.3 0.25 0.2 15 20 Concentration ug /ml 25 10 Absorbance
3. In Part A, you measure the absorbance of four FesCN" solutions and prepare a plot (Absorbance vs. concentration) similar to the one below. Beer's Law Plot for FeSCN2 0.6 0.5 0.4 0.3 d 0.2 0.1 0 y = 2417.5× + 0.0483 R2 0.99897 0.00025 0.00015 0.0002 0.00005 0.0001 0 Concentration (M) You use this plot as a standard curve in Part B to determine the concentration of an unknown. If an unknown FeSCN2 solution gives an absorbance reading of...
Calibration curve of dilutions 0.6 0.5 y = 8E-05x2 + 0.0189x R2 = 0.99615 0.4 Absorbance 0.3 0.2 0.1 0 0 5 10 15 20 25 30 Concentration Using this calibration curve, what is the concentration of the sample if the sample has its Absorbance 86.22 HTML Editor
1. A researcher was evaluating the concenltaIO U a submerged fermentation. He prepared a stock solution of 0.1mg bovine Serum Albumin. Plot a graph of a standard curve using the following data sets and determine the concentration of the following proteins X, Y, and Z Concentration mg/ml 0.2 0.4 0.6 0.8 1.0 Absorbance nm 0.245 0.389 0.612 0.745 1.025 0.824 0.452 0.689 Convert the concentration of X, Y, and Z to g/l and %w/v.
1. A researcher was evaluating the...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
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