Clamorosus, sordidus and manus parvus are recessive traits located on chromosome 3 in the newly-discovered diploid species electio vigintiviginti. The genetic map of this region is cl-8-s-10-mp. If you performed a test-cross between a fully heterozygous female and a fully homozygous male and observed 500 progeny, how many phenotypic classes of progeny would be expected and in what numbers?
Answer:
cl----8 mu----s----10mu---mp
Distance between the genes (mu) = (%) of recombination frequency
Expected double crossover frequency = 8% x 10% = 0.008 or 0.8%
Explanation:
|
Class of gametes |
Genotype |
Frequency of reciprocal pair |
Numbers |
Frequency of each class |
% of frequency = frequency * 1000 |
|
Parental |
+ + + |
100- all recombinant progeny |
100%-(7.2%+9.2%+0.8%) = 82.8% |
41.4% |
41.4%*500 = 207 |
|
cl s mp |
41.4% |
41.4%*500 = 207 |
|||
|
SCO 1 |
+ s mp |
RF in region 1 = SCO1 – DCO |
SCO1 = 8% - 0.8% = 7.2% |
3.6% |
3.6% * 500 = 18 |
|
cl + + |
3.6% |
3.6% * 500 = 18 |
|||
|
SCO 2 |
+ + mp |
RF in region 2 = SCO2 - DCO |
SCO2 = 10% – 0.8% = 9.2% |
4.6% |
4.6% * 500 = 23 |
|
cl s + |
4.6% |
4.6% * 500 = 23 |
|||
|
DCO |
+ s + |
(RF in region 1) * (RF in region 2) |
0.8% |
0.4% |
0.4% *500 = 2 |
|
cl + mp |
0.4% |
0.4% *500 = 2 |
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