Question

A: The gene that codes for the rho protein in a specific species of bacteria is deleted. What function is likely lost because of this deletion? Be specific.

B: What role does the TBP (TATA-Binding Protein) play in transcriptional initiation?

C: What is the purpose of having a 5’ CAP on eukaryotic mRNA?

D: Where do processed mRNA’s go after leaving the nucleus and how?

Please answer and explain

E: Explain the role of snRNPs in RNA processing. Be specific.

Answer 1

Date: Page no: Anuwen Rho gene Rho protein hexmen of single polypeptide (14909) Rho protein haave Capability to bind with RNA so it is RNA binding protein with the capacity To hy holyze ATP and nucleotide triphophate reach subunit have both Capasity (Alp hy drodze and nucleotile triphoophate). Rho protein is an Arp dependent RNA .shimulated heliade that distrupts The nascent RNA-DNA Complex Binding site of Rho protein on RNA is "gut" site. eich in e residue ③ role of TBP (TATA Binding protein ) TBP Transcription factor ao made by two component ITBP Top aboo ciated factes (TAFO ir is sequence specific protein and bind with DNA via TBp demain jt is Bind with minor groove in the region Istrara box Sale it is requhed for initiation of Transcription by I RNA polymerase I and a & it is couNe DNA banding

Date: Page no: o purpose of having a s' cap on miRNA 5 Cap is a part of RNA processing many enzyme are helpful en processing like huany transferage" » $6P PPve MRAD punpowe Because so many protection of mRNA from degradation (txo) M. RNA Travel from nucleou to cytoplasm EXO / Rendo nuclease knzyme are pe Cytor Topon for helpful in Transport of the morng from Nuclear Binding of Ribosome with M. RNA Trandation t M-RNA T Translation protein Cytoplacom mRNA nucleup Cell atten procearing of mRNA it transported from nudeos To cytoplasm via nuclear pore & parin protein). only in Peton cy to placem m-RNA go for splicing and after Translation

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