Question

How is initiation at oriC regulated in bacteria?

DnaA-ATP cannot bind to a fully methylated origin of replication.

DnaA-ADP cannot convert the DNA unwinding element from closed to open.

DnaB must be phophorylated by cyclin dependent kinases.

All of the above are mechanisms of regulating initiation in bacteria.

Answer 1

Answer :) all options

The replication origin (OriC) is controlled by specific oriC-binding proteins and DNA methylation. DnaA contains DNA-binding and ATP/ADP-binding domains. In the presence of enough ATP-DnaA amount in the cell, the initiation of the OriC opening starts. DNA methylation protects the binding of DNA binding proteins. DnaB (Helicase) is required to be phosphorylated before binding to the DNA and then it unwinds the DNA. DnaA-ADP is made by the hydrolysis of DnaA-ATP and gives negative feedback for the regulation of OriC.

Therefore, all options are correct.

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